{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:43:55Z","timestamp":1792122235650,"version":"4.4.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2011,7,15]],"date-time":"2011-07-15T00:00:00Z","timestamp":1310688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Although live-attenuated influenza vaccines (LAIV) are safe for use in protection against seasonal influenza strains, concerns regarding their potential to reassort with wild-type virus strains have been voiced. LAIVs have been demonstrated to induce enhanced mucosal and cell-mediated immunity better than inactivated vaccines while also requiring a smaller dose to achieve a protective immune response. To address the need for a reassortment-incompetent live influenza A virus vaccine, we have designed a chimeric virus that takes advantage of the fact that influenza A and B viruses do not reassort. Our novel vaccine prototype uses an attenuated influenza B virus that has been manipulated to express the ectodomain of the influenza A hemagglutinin protein, the major target for eliciting neutralizing antibodies. The hemagglutinin RNA segment is modified such that it contains influenza B packaging signals, and therefore it cannot be incorporated into a wild-type influenza A virus. We have applied our strategy to different influenza A virus subtypes and generated chimeric B\/PR8 HA (H1), HK68 (H3), and VN (H5) viruses. All recombinant viruses were attenuated both\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            , and immunization with these recombinant viruses protected mice against lethal influenza A virus infection. Overall, our data indicate that the chimeric live-attenuated influenza B viruses expressing the modified influenza A hemagglutinin are effective LAIVs.\n          <\/jats:p>","DOI":"10.1128\/jvi.00609-11","type":"journal-article","created":{"date-parts":[[2011,5,5]],"date-time":"2011-05-05T03:33:06Z","timestamp":1304566386000},"page":"6832-6843","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains"],"prefix":"10.1128","volume":"85","author":[{"given":"Rong","family":"Hai","sequence":"first","affiliation":[{"name":"Department of Microbiology, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo","family":"Garc\u00eda-Sastre","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York"},{"name":"Institute of Global Health and Emerging Pathogens, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York"},{"name":"Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Swayne","sequence":"additional","affiliation":[{"name":"USDA-ARS, Southeast Poultry Research Laboratory, Exotic and Emerging Avian Viral Diseases Research Unit, Athens, Georgia 30605-2195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Palese","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York"},{"name":"Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.031575198"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00471-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)11212-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000409"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.17.9116-9123.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9679-9682.1999"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308352100"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9936"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01213-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.14.8031-8038.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1353\/bhm.2002.0022"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-64-10-2139"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/314713"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01318082"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01305-10"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/471157a"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1201.051043"},{"key":"e_1_3_2_19_2","first-page":"1647","volume-title":"Fields virology","author":"Palese P.","year":"2007","unstructured":"PaleseP. 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