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An off-label use of the purine nucleoside analogue ribavirin (1-\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -\n                    <jats:sc>d<\/jats:sc>\n                    -ribofuranosyl-1-\n                    <jats:italic>H<\/jats:italic>\n                    -1,2,4-triazole-3-carboxamide) is the only antiviral treatment currently available for arenavirus infections. However, the ribavirin antiviral mechanism action against arenaviruses remains unknown. Here we document that ribavirin is mutagenic for the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) in cell culture. The mutagenic activity of ribavirin on LCMV was observed under single- and multiple-passage regimes and could not be accounted for by a decrease of the intracellular GTP pool promoted by ribavirin-mediated inhibition of inosine monophosphate dehydrogenase (IMPDH). Our findings suggest that the antiviral activity of ribavirin on arenaviruses might be exerted, at least partially, by lethal mutagenesis. Implications for antiarenavirus therapy are discussed.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00614-11","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T00:29:37Z","timestamp":1305160177000},"page":"7246-7255","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Ribavirin Can Be Mutagenic for Arenaviruses"],"prefix":"10.1128","volume":"85","author":[{"given":"H\u00e9ctor","family":"Moreno","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas, Campus de Cantoblanco, 28049 Madrid, Spain"},{"name":"Department of Neuropharmacology, The Scripps Research Institute, IMM-6, 10550 North Torrey Pines Road, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Gallego","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas, Campus de Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noem\u00ed","family":"Sevilla","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, Instituto Nacional de Investigaci\u00f3n Agraria y Alimentaria, Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"de la Torre","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, The Scripps Research Institute, IMM-6, 10550 North Torrey Pines Road, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Domingo","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas, Campus de Cantoblanco, 28049 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas, Campus de Cantoblanco, 28049 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, Instituto Nacional de Investigaci\u00f3n Agraria y Alimentaria, Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.03.014"},{"key":"e_1_3_2_3_2","first-page":"652","article-title":"Molecular characterization of a dual inhibitory and mutagenic activity of 5-fluorouridine triphosphate on viral RNA synthesis","volume":"382","author":"Agudo R.","year":"2008","unstructured":"AgudoR.. 2008. 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