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In this study, we developed a new reverse genetics system for HCV infection based on\n            <jats:italic>trans<\/jats:italic>\n            -complementation of viral structural proteins (HCV\u0394S). This system can be used to display structural proteins of HCV strains of multiple genotypes as well as clinical isolates. By using this system, we showed that multiple different HCV structural proteins from a same patient were displayed on HCV\u0394S. Interestingly, these variant structural proteins within the same patient can be classified according to the sequence of HVR1in E2, which dictates viral sensitivity to nAbs and viral evolution\n            <jats:italic>in vivo<\/jats:italic>\n            . Our work provided a new tool to study highly divergent HCV quasispecies and shed light on underlying mechanisms driving HCV evolution.\n          <\/jats:p>","DOI":"10.1128\/jvi.00622-20","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T13:00:01Z","timestamp":1592485201000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["A Novel Approach To Display Structural Proteins of Hepatitis C Virus Quasispecies in Patients Reveals a Key Role of E2 HVR1 in Viral Evolution"],"prefix":"10.1128","volume":"94","author":[{"given":"Yimin","family":"Tong","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingchao","family":"Li","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of 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