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In fact, only a few viral antigens are detected in bronchial epithelial cells from autopsied lung sections. Previously, we detected many viral antigens in AEC-IIs from the lung. This was because the majority of A\/H1N1\/pdm09 in the lung tissue harbored an aspartic acid-to-glycine substitution at position 222 (D222G) of the hemagglutinin protein. A\/H1N1\/pdm09 harboring the D222G substitution has a receptor-binding preference for \u03b1-2,3-linked sialic acids expressed on human AECs and infects them in the same way as H5N1 and H7N9 avian IAVs. Here, we report the first successful observation of virus particles, not only in AEC-IIs, but also in Ms\/M\u03d5s and Neus, using electron microscopy. The finding of a M\/M\u03d5 harboring numerous virus particles within vesicles and at the cell surface suggests that Ms\/M\u03d5s are involved in the pathogenesis of IAV primary pneumonia.<\/jats:p>","DOI":"10.1128\/jvi.00644-19","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T11:38:31Z","timestamp":1562758711000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Serial Section Array Scanning Electron Microscopy Analysis of Cells from Lung Autopsy Specimens following Fatal A\/H1N1 2009 Pandemic Influenza Virus Infection"],"prefix":"10.1128","volume":"93","author":[{"given":"Michiyo","family":"Kataoka","sequence":"first","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinji","family":"Ishida","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University, Iwate, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsutoshi","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University, Iwate, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Nozaki","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University, Iwate, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoh-Ichi","family":"Satoh","sequence":"additional","affiliation":[{"name":"Department of Medical Education, Iwate Medical University, Iwate, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsutaro","family":"Sata","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"},{"name":"Global Virus Network, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00269"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.05.010"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/440435a"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0904252"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.15.14.19534-en"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.01.013"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12146"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.7883\/yoken.63.67","article-title":"The first autopsy case of pandemic influenza (A\/H1N1pdm) virus infection in Japan: detection of a high copy number of the virus in type II alveolar epithelial cells by pathological and virological examination","volume":"63","author":"Nakajima N","year":"2010","unstructured":"Nakajima N, Hata S, Sato Y, Tobiume M, Katano H, Kaneko K, Nagata N, Kataoka M, Ainai A, Hasegawa H, Tashiro M, Kuroda M, Odai T, Urasawa N, Ogino T, Hanaoka H, Watanabe M, Sata T. 2010. 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