{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:49:47Z","timestamp":1792097387532,"version":"4.4.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2010,9,15]],"date-time":"2010-09-15T00:00:00Z","timestamp":1284508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enteroviruses (\n            <jats:italic>Picornaviridae<\/jats:italic>\n            family) are a common cause of human illness worldwide and are associated with diverse clinical syndromes, including asymptomatic infection, respiratory illness, gastroenteritis, and meningitis. In this study, we report the identification and complete genome sequence of a novel enterovirus isolated from a case of acute respiratory illness in a Nicaraguan child. Unbiased deep sequencing of nucleic acids from a nose and throat swab sample enabled rapid recovery of the full-genome sequence. Phylogenetic analysis revealed that human enterovirus 109 (EV109) is most closely related to serotypes of human enterovirus species C (HEV-C) in all genomic regions except the 5\u2032 untranslated region (5\u2032 UTR). Bootstrap analysis indicates that the 5\u2032 UTR of EV109 is likely the product of an interspecies recombination event between ancestral members of the HEV-A and HEV-C groups. Overall, the EV109 coding region shares 67 to 72% nucleotide sequence identity with its nearest relatives. EV109 isolates were detected in 5\/310 (1.6%) of nose and throat swab samples collected from children in a pediatric cohort study of influenza-like illness in Managua, Nicaragua, between June 2007 and June 2008. Further experimentation is required to more fully characterize the pathogenic role, disease associations, and global distribution of EV109.\n          <\/jats:p>","DOI":"10.1128\/jvi.00698-10","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T02:14:51Z","timestamp":1277950491000},"page":"9047-9058","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":120,"title":["Human Enterovirus 109: a Novel Interspecies Recombinant Enterovirus Isolated from a Case of Acute Pediatric Respiratory Illness in Nicaragua"],"prefix":"10.1128","volume":"84","author":[{"given":"Nathan L.","family":"Yozwiak","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Skewes-Cox","sequence":"additional","affiliation":[{"name":"Biological and Medical Informatics Program"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aubree","family":"Gordon","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California"},{"name":"Fogarty International Center, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saira","family":"Saborio","sequence":"additional","affiliation":[{"name":"Departamento de Virologi\u0301a, Centro Nacional de Diagnostico y Referencia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermina","family":"Kuan","sequence":"additional","affiliation":[{"name":"Centro de Salud So\u0301crates Flores Vivas, Ministerio de Salud, Managua, Nicaragua"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Balmaseda","sequence":"additional","affiliation":[{"name":"Departamento de Virologi\u0301a, Centro Nacional de Diagnostico y Referencia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don","family":"Ganem","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute"},{"name":"Medicine, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Harris","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph L.","family":"DeRisi","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute"},{"name":"Departments of Biochemistry"},{"name":"Medicine, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(03)00163-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80164-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(02)00019-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.7872"},{"key":"e_1_3_2_6_2","first-page":"127","volume":"19","year":"2005","unstructured":"Bolanaki, E., C. 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