{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T02:14:25Z","timestamp":1782526465365,"version":"3.54.5"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MOST | National Key Research and Development Program of China","award":["2021YFD1800100"],"award-info":[{"award-number":["2021YFD1800100"]}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31941002"],"award-info":[{"award-number":["31941002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31872448"],"award-info":[{"award-number":["31872448"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"crossref","award":["YQ2019C033"],"award-info":[{"award-number":["YQ2019C033"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2023,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            African swine fever is a contagious and lethal disease of domestic pigs and wild boars, which has caused significant economic loss for the swine industry. African swine fever virus (ASFV) is the etiological agent of ASF. Until now, no effective commercial vaccine and antiviral drugs are available for ASF control. Here, we report a new designed live-attenuated mutant (ASFV-\u0394H240R-\u03947R) by deleting H240R and MGF505-7R genes based on the highly pathogenic ASFV HLJ\/18 backbone. The viral titer of ASFV-\u0394H240R-\u03947R was decreased approximately 1.0 log in porcine alveolar macrophages (PAMs) compared with its parental ASFV HLJ\/18, and it is highly stability following 30 serial passages\n            <jats:italic>in vitro<\/jats:italic>\n            . Piglets immunized by intramuscular inoculation with 10\n            <jats:sup>3<\/jats:sup>\n            or 10\n            <jats:sup>5<\/jats:sup>\n            HAD\n            <jats:sub>50<\/jats:sub>\n            of ASFV-\u0394H240R-\u03947R displayed safety without any ASF-related signs and could not be transmitted by direct contact. In addition, the immunized pigs produced specific antibodies against p30 but not those cohabitation piglets. Challenged with a virulent ASFV isolate HLJ\/18, the piglets in the immunized group with 10\n            <jats:sup>3<\/jats:sup>\n            HAD\n            <jats:sub>50<\/jats:sub>\n            of ASFV-\u0394H240R-\u03947R obtained clinically significant 100% protection with mild clinical symptoms, whereas the piglets in the immunized group with 10\n            <jats:sup>5<\/jats:sup>\n            HAD\n            <jats:sub>50<\/jats:sub>\n            of ASFV-\u0394H240R-\u03947R obtained 100% protection without clinical symptoms. Moreover, the piglets in the immunized group with 10\n            <jats:sup>5<\/jats:sup>\n            HAD\n            <jats:sub>50<\/jats:sub>\n            of ASFV-\u0394H240R-\u03947R exhibited low levels of virus replication and with no observed pathological changes by postmortem and histological analysis. Overall, our results provided a new designed live vaccine candidate and rationalized the understanding of ASFV gene function, virus attenuation, and protection against ASFV infection.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>African swine fever (ASF) caused by ASF virus (ASFV) is a highly contagious and acute hemorrhagic viral disease in domestic pigs. Until now, no effective commercial vaccine and antiviral drugs are available for ASF control. Here, we generated a new live-attenuated vaccine candidate (ASFV-\u0394H240R-\u03947R) by deleting H240R and MGF505-7R genes from the highly pathogenic ASFV HLJ\/18 genome. Piglets immunized with ASFV-\u0394H240R-\u03947R were safe without any ASF-related signs and produced specific antibodies against p30. Challenged with a virulent ASFV HLJ\/18, the piglets immunized with high-dose group (105 HAD50) exhibited 100% protection without clinical symptoms, showing that low levels of virus replication with no observed pathogenicity by postmortem and histological analysis. Overall, our results provided a new strategy by designing live-attenuated vaccine candidate, resulting in protection against ASFV infection.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jvi.00704-23","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T13:17:51Z","timestamp":1695907071000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain"],"prefix":"10.1128","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7984-9498","authenticated-orcid":false,"given":"Jiangnan","family":"Li","sequence":"first","affiliation":[{"name":"Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China"},{"name":"Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Song","sequence":"additional","affiliation":[{"name":"Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China"},{"name":"Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China"},{"name":"Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China"},{"name":"Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease 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