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Development of novel vaccine platforms against these pathogens is a public health priority. Using WNV as a model, we developed a new vaccine platform for flaviviruses. WNV containing a NS1 deletion (WNV-\u0394NS1) could be efficiently\n            <jats:italic>trans<\/jats:italic>\n            complemented in Vero cells that constitutively expressed WT NS1 protein. A single-dose immunization with WNV-\u0394NS1 elicited robust immune responses in mice. The immunized animals were fully protected against pathogenic WNV infection. No adverse effects related to the WNV-\u0394NS1 vaccination were observed. The results have demonstrated the potential of the NS1 complementation system as an alternative platform for flavivirus vaccine development, especially for highly pathogenic flaviviruses.\n          <\/jats:p>","DOI":"10.1128\/jvi.00720-19","type":"journal-article","created":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T13:28:02Z","timestamp":1560432482000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Replication-Defective West Nile Virus with NS1 Deletion as a New Vaccine Platform for Flavivirus"],"prefix":"10.1128","volume":"93","author":[{"given":"Na","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China"},{"name":"University of Chinese Academy of Sciences, Beijing, 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