{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:24:13Z","timestamp":1790763853109,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100010767","name":"Innovative Medicines Initiative","doi-asserted-by":"publisher","award":["ZAPI 115760"],"award-info":[{"award-number":["ZAPI 115760"]}],"id":[{"id":"10.13039\/501100010767","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19AI135972"],"award-info":[{"award-number":["U19AI135972"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GSC-4"],"award-info":[{"award-number":["GSC-4"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,8,17]]},"abstract":"<jats:p>\n            SBV is a newly emerging orthobunyavirus (family\n            <jats:italic>Peribunyaviridae<\/jats:italic>\n            ) that has spread rapidly across Europe since 2011, resulting in substantial economic losses in livestock farming. In this study, we performed unbiased genome-wide CRISPR-Cas9 screening and identified PAPST1, a sulfotransferase encoded by\n            <jats:italic>SLC35B2<\/jats:italic>\n            , as a host entry factor for SBV. Consistent with its role in the synthesis of heparan sulfate, we show that this activity is required for efficient infection by SBV. A comparable dependency on heparan sulfate was also observed for La Crosse virus and Rift Valley fever virus, highlighting the importance of heparan sulfate for host cell infection by bunyaviruses. Thus, the present work provides crucial insights into virus-host interactions of important animal and human pathogens.\n          <\/jats:p>","DOI":"10.1128\/jvi.00752-20","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T13:33:02Z","timestamp":1591795982000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["A Genome-Wide CRISPR-Cas9 Screen Reveals the Requirement of Host Cell Sulfation for Schmallenberg Virus Infection"],"prefix":"10.1128","volume":"94","author":[{"given":"Thiprampai","family":"Thamamongood","sequence":"first","affiliation":[{"name":"Institute of Virology, Medical Center-University of Freiburg, Freiburg, Germany"},{"name":"Faculty of Medicine, University of Freiburg, Freiburg, Germany"},{"name":"Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany"},{"name":"Faculty of Biology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Aebischer","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Center-University of Freiburg, Freiburg, Germany"},{"name":"Faculty of Medicine, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max W.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Elling","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine, Medical Center-University of Freiburg, Freiburg, Germany"},{"name":"Institute for Immunodeficiency, Center for Chronic Immunodeficiency, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Benner","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo","family":"Garc\u00eda-Sastre","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA"},{"name":"Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA"},{"name":"Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA"},{"name":"The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0187-559X","authenticated-orcid":false,"given":"Georg","family":"Kochs","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Center-University of Freiburg, Freiburg, Germany"},{"name":"Faculty of Medicine, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Beer","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2972-6855","authenticated-orcid":false,"given":"Martin","family":"Schwemmle","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Center-University of Freiburg, Freiburg, Germany"},{"name":"Faculty of Medicine, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1803.111905"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08832-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812002245"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2014.03.021"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001365"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200103153441103"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2014.08.033"},{"key":"e_1_3_2_10_2","first-page":"281","volume-title":"The viruses","author":"Morrill JC","year":"1996","unstructured":"Morrill JC, McClain DJ. 1996. Epidemiology and pathogenesis of Rift Valley fever and other phleboviruses, p 281\u2013293. In Elliott RM (ed), The Bunyaviridae. The viruses. Springer, Boston, MA."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2006.06.005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir804"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3332"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.021774-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4682-1-10"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1017"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(83)90182-4"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3810159"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90889-j"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00503-17"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0955-3"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3741"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0121-3"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2019.11.005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18631"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.06.028"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1900867116"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3437"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0554-4"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwq154"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302439200"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.63.1.52-58.1989"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.116.5.1273"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.065201-0"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep11346"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110617108"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01364-12"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02055-15"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.22.14451-14456.2005"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302267200"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007123"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120244109"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-23056-1_2"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003374"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.013540-0"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.2.1092-1104.2003"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247005"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a120512"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.2008\/002097-0"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2012.9"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0165-4"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-11-2683"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.03.035"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00752-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00752-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:11:07Z","timestamp":1646493067000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00752-20"}},"subtitle":[],"editor":[{"given":"Stacey","family":"Schultz-Cherry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,8,17]]},"references-count":52,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2020,8,17]]}},"alternative-id":["10.1128\/JVI.00752-20"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00752-20","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,17]]},"assertion":[{"value":"2020-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00752-20"}}