{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:27:52Z","timestamp":1790458072519,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T00:00:00Z","timestamp":1568505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000185","name":"DOD | Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["HR001118S0017-PREEMPT-FP001"],"award-info":[{"award-number":["HR001118S0017-PREEMPT-FP001"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,9,15]]},"abstract":"<jats:p>Chikungunya fever is a debilitating disease that causes severe pain to the joints, which can compromise the patient\u2019s lifestyle for several months and even in some grave cases lead to death. The etiological agent is chikungunya virus, an alphavirus transmitted by mosquito bite. Currently, there are no approved vaccines or treatments against the disease. In our research, we developed novel live attenuated vaccine candidates against chikungunya virus by applying an innovative genomic design. When tested in the insect and mammalian host, the vaccine candidates did not cause disease, elicited strong protection against further infection, and had low risk of reversion to pathogenic phenotypes.<\/jats:p>","DOI":"10.1128\/jvi.00775-19","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T09:34:53Z","timestamp":1561714493000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Chikungunya Virus Vaccine Candidates with Decreased Mutational Robustness Are Attenuated<i>In Vivo<\/i>and Have Compromised Transmissibility"],"prefix":"10.1128","volume":"93","author":[{"given":"Luc\u00eda","family":"Carrau","sequence":"first","affiliation":[{"name":"Viral Populations and Pathogenesis Unit, Department of Virology, Institut Pasteur, CNRS UMR 3569, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica V.","family":"Rezelj","sequence":"additional","affiliation":[{"name":"Viral Populations and Pathogenesis Unit, Department of Virology, Institut Pasteur, CNRS UMR 3569, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Noval","sequence":"additional","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura I.","family":"Levi","sequence":"additional","affiliation":[{"name":"Viral Populations and Pathogenesis Unit, Department of Virology, Institut Pasteur, CNRS UMR 3569, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Megrian","sequence":"additional","affiliation":[{"name":"Evolutionary Biology of the Microbial Cell Unit, Department of Microbiology, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve","family":"Blanc","sequence":"additional","affiliation":[{"name":"Viral Populations and Pathogenesis Unit, Department of Virology, Institut Pasteur, CNRS UMR 3569, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Weger-Lucarelli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Moratorio","sequence":"additional","affiliation":[{"name":"Viral Populations and Pathogenesis Unit, Department of Virology, Institut Pasteur, CNRS UMR 3569, Paris, France"},{"name":"Laboratorio de Inmunovirolog\u00eda, Institut Pasteur de Montevideo, Montevideo, Uruguay"},{"name":"Laboratorio de Virolog\u00eda Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth A.","family":"Stapleford","sequence":"additional","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4400-771X","authenticated-orcid":false,"given":"Marco","family":"Vignuzzi","sequence":"additional","affiliation":[{"name":"Viral Populations and Pathogenesis Unit, Department of Virology, Institut Pasteur, CNRS UMR 3569, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2014.1745"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(55)90081-x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400044454"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004101"},{"key":"e_1_3_3_6_2","unstructured":"CDC. 2018. Chikungunya virus: geographic distribution. https:\/\/www.cdc.gov\/chikungunya\/geo\/index.html."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2368"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2017.03.006"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00104-16"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.62.681"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2012.1.1.35"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1006"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.09.007"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00738-06"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.7.3259-3272.2006"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1155761"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.04531"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00508-09"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.88"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.10.008"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.11.002"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1554\/02-750R"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/82191"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2004.11.008"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","first-page":"846","DOI":"10.4049\/jimmunol.160.2.846","article-title":"In vitro and in vivo mechanisms of action of the antiproliferative and immunosuppressive agent, brequinar sodium","volume":"160","author":"Xu X","year":"1998","unstructured":"Xu X, Williams JW, Shen J, Gong H, Yin D-P, Blinder L, Elder RT, Sankary H, Finnegan A, Chong A-F. 1998. In vitro and in vivo mechanisms of action of the antiproliferative and immunosuppressive agent, brequinar sodium. J Immunol 160:846\u2013853.","journal-title":"J Immunol"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003678"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2009.45.7.1384878"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02603-09"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.05.008"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-101416-041757"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08347"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.4081\/gh.2014.29"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2017.11.026"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.10.008"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1348(03)00004-2"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.84"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002390"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01943-14"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201200213"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.034884"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-001-0064-3"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a004059"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.20.11296-11298.2003"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81015-0"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712130105"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.139"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/v2122594"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030263"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.11.002"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2018.06.004"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_3_54_2","unstructured":"Vignuzzi M Barbezange C Moratorio G. 19 February 2019. RNA virus attenuation by alteration of mutational robustness and sequence space. US patent 10 206 994."}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00775-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00775-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T20:58:45Z","timestamp":1694984325000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00775-19"}},"subtitle":[],"editor":[{"given":"Terence S.","family":"Dermody","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,9,15]]},"references-count":53,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2019,9,15]]}},"alternative-id":["10.1128\/JVI.00775-19"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00775-19","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,15]]},"assertion":[{"value":"2019-05-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00775-19"}}