{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:39:53Z","timestamp":1778636393124,"version":"3.51.4"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFC2604100, 2021YFC2301400, 2022YFC2304102"],"award-info":[{"award-number":["2022YFC2604100, 2021YFC2301400, 2022YFC2304102"]}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["92269203, 81971501"],"award-info":[{"award-number":["92269203, 81971501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Tthe National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82302519"],"award-info":[{"award-number":["82302519"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Units of Adaptive Evolution and Control of Emerging Viruses, Chinese Academy of Medical Sciences","award":["2018RU009"],"award-info":[{"award-number":["2018RU009"]}]},{"name":"Excellent Young Scientist Program of the NSFC","award":["81822040"],"award-info":[{"award-number":["81822040"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2024,1,23]]},"abstract":"ABSTRACT<\/jats:title>\n \n Zika virus (ZIKV) infection caused neurological complications and male infertility, leading to the accumulation of antigen-specific immune cells in immune-privileged organs (IPOs). Thus, it is important to understand the immunological responses to ZIKV in IPOs. We extensively investigated the ZIKV-specific T cell immunity in IPOs in\n Ifnar1<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mice, based on an immunodominant epitope E\n 294-302<\/jats:sub>\n tetramer. The distinct kinetics and functions of virus-specific CD8\n +<\/jats:sup>\n T cells infiltrated into different IPOs were characterized, with late elevation in the brain and spinal cord. Single epitope E\n 294-302<\/jats:sub>\n -specific T cells can account for 20-60% of the total CD8\n +<\/jats:sup>\n T cells in the brain, spinal cord, and testicle and persist for at least 90 days in the brain and spinal cord. The E\n 294-302<\/jats:sub>\n -specific TCR\u03b1\u03b2s within the IPOs are featured with the majority of clonotypes utilizing\n TRAV9N-3<\/jats:italic>\n paired with diverse TRBV chains, but with distinct \u03b1\u03b2 paired clonotypes in 7 and 30 days post-infection. Specific chemokine receptors,\n Ccr2<\/jats:italic>\n and\n Ccr5<\/jats:italic>\n , were selectively expressed in the E\n 294-302<\/jats:sub>\n -specific CD8\n +<\/jats:sup>\n T cells within the brain and testicle, indicating an IPO-oriented migration of virus-specific CD8\n +<\/jats:sup>\n T cells after infection. Overall, this study adds to the understanding of virus-specific CD8\n +<\/jats:sup>\n T cell responses for controlling and clearing ZIKV infection in IPOs.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n The immune-privileged organs (IPOs), such as the central nervous system and testicles, presented pathogenicity and inflammation after Zika virus (ZIKV) infection with infiltrated CD8\n +<\/jats:sup>\n T cells. Our data show that CD8\n +<\/jats:sup>\n T cells keep up with virus increases and decreases in immune-privileged organs. Furthermore, our study provides the first\n ex vivo<\/jats:italic>\n comparative analyses of the composition and diversity related to TCR\u03b1\/\u03b2 clonotypes across anatomical sites and ZIKV infection phases. We show that the vast majority of TCR\u03b1\/\u03b2 clonotypes in tissues utilize\n TRAV9N-3<\/jats:italic>\n with conservation. Specific chemokine expression, including\n Ccr2<\/jats:italic>\n and\n Ccr5<\/jats:italic>\n , was found to be selectively expressed in the E\n 294-302<\/jats:sub>\n -specific CD8\n +<\/jats:sup>\n T cells within the brain and testicle, indicating an IPO-oriented migration of the virus-specific CD8\n +<\/jats:sup>\n T cells after the infection. Our study adds insights into the anti-viral immunological characterization and chemotaxis mechanism of virus-specific CD8\n +<\/jats:sup>\n T cells after ZIKV infection in different IPOs.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/jvi.00789-23","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T14:00:57Z","timestamp":1704290457000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of CD8\n +<\/sup>\n T cells in immune-privileged organs of ZIKV-infected\n Ifnar1<\/i>\n \u2212\/\u2212<\/sup>\n mice"],"prefix":"10.1128","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7334-2536","authenticated-orcid":false,"given":"Hangjie","family":"Zhang","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Biosafety, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID), National Institute for Viral 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