{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:36:30Z","timestamp":1790724990034,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Guangdong Provincial Novel Coronavirus Scientific and Technological Project","award":["2020111107001"],"award-info":[{"award-number":["2020111107001"]}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["203141\/Z\/16\/Z"],"award-info":[{"award-number":["203141\/Z\/16\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012245","name":"GDSTC | Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["2018B0202007006"],"award-info":[{"award-number":["2018B0202007006"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,8,17]]},"abstract":"<jats:p>\n                    The spike protein determines the infectivity and host range of coronaviruses. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has two unique features in its spike protein, the receptor binding domain and an insertion of 12 nucleotides at the S1\/S2 boundary resulting in a furin-like cleavage site. Here, we identified two deletion variants of SARS-CoV-2 that either directly affect the furin-like cleavage site itself (NSPRRAR) or a flanking sequence (QTQTN), and we investigated these deletions in cell isolates and clinical samples. The absence of the polybasic cleavage site in SARS-CoV-2 did not affect virus replication in Vero or Vero-E6 cells. Our data indicate the PRRAR sequence and the flanking QTQTN sequence are not fixed\n                    <jats:italic>in vitro;<\/jats:italic>\n                    thus, there appears to be distinct selection pressures on SARS-CoV-2 sequences\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Further investigation of the mechanism of generating these deletion variants and their infectivity in different animal models would improve our understanding of the origin and evolution of this virus.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00790-20","type":"journal-article","created":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T10:29:25Z","timestamp":1592908165000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":129,"title":["Identification of Common Deletions in the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus 2"],"prefix":"10.1128","volume":"94","author":[{"given":"Zhe","family":"Liu","sequence":"first","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifang","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2072-0721","authenticated-orcid":false,"given":"Runyu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinju","family":"Peng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4882-0695","authenticated-orcid":false,"given":"Qianling","family":"Xiong","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiufeng","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1842-316X","authenticated-orcid":false,"given":"Baisheng","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1122-6999","authenticated-orcid":false,"given":"Lina","family":"Yi","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Peng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institution of Public Health, Guangzhou, China"},{"name":"Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben J. 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