{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:06:18Z","timestamp":1790831178661,"version":"4.1.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,9,15]],"date-time":"2018-09-15T00:00:00Z","timestamp":1536969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Idex University of Bordeaux","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KE 660\/16-1"],"award-info":[{"award-number":["KE 660\/16-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR 14 IFEC 0003-04"],"award-info":[{"award-number":["ANR 14 IFEC 0003-04"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["2013-ANDY"],"award-info":[{"award-number":["2013-ANDY"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la Recherche sur le Cancer","doi-asserted-by":"publisher","award":["Fellowship"],"award-info":[{"award-number":["Fellowship"]}],"id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2018,9,15]]},"abstract":"<jats:p>\n            Viruses must deliver their genomes to host cells to ensure replication and propagation. Characterizing the fate of viral genomes is crucial to understand the viral life cycle and the fate of virus-derived vector tools. Here, we integrated the ANCHOR3 system, an\n            <jats:italic>in vivo<\/jats:italic>\n            DNA-tagging technology, into the adenoviral genome for real-time genome detection. ANCHOR3 tagging permitted the\n            <jats:italic>in vivo<\/jats:italic>\n            visualization of incoming genomes at the onset of infection and of replicated genomes at late phases of infection. Using this system, we show viral genome attachment to condensed host chromosomes during mitosis, identifying this mechanism as a mode of cell-to-cell transfer. We characterize the spatiotemporal organization of adenovirus replication and identify two kinetically distinct phases of viral genome replication. The ANCHOR3 system is the first technique that allows the continuous visualization of adenoviral genomes during the entire virus life cycle, opening the way for further in-depth study.\n          <\/jats:p>","DOI":"10.1128\/jvi.00795-18","type":"journal-article","created":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T10:44:13Z","timestamp":1531133053000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["<i>In Vivo<\/i>\n            Labelling of Adenovirus DNA Identifies Chromatin Anchoring and Biphasic Genome Replication"],"prefix":"10.1128","volume":"92","author":[{"given":"Tetsuro","family":"Komatsu","sequence":"first","affiliation":[{"name":"CNRS UMR 5234, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Quentin-Froignant","sequence":"additional","affiliation":[{"name":"NeoVirTech SAS, ITAV, Universit\u00e9 de Toulouse, CNRS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Carlon-Andres","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Floriane","family":"Lagadec","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, Universit\u00e9 de Bordeaux, Bordeaux, France"},{"name":"Department of Molecular Biology, Faculty of Medicine, G\u00f6ttingen Center of Biosciences (GZMB), Georg-August-University G\u00f6ttingen, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Rayne","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Ragues","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph H.","family":"Kehlenbach","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Faculty of Medicine, G\u00f6ttingen Center of Biosciences (GZMB), Georg-August-University G\u00f6ttingen, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chair for Virology and Microbiology, Witten\/Herdecke University, Witten, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Ehrhardt","sequence":"additional","affiliation":[{"name":"Chair for Virology and Microbiology, Witten\/Herdecke University, Witten, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Bystricky","sequence":"additional","affiliation":[{"name":"LBME, Centre de Biologie Int\u00e9grative, CBI, Universit\u00e9 de Toulouse, CNRS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud","family":"Morin","sequence":"additional","affiliation":[{"name":"IMACTIV3D SAS, ITAV, Universit\u00e9 de Toulouse, CNRS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Lagarde","sequence":"additional","affiliation":[{"name":"IMACTIV3D SAS, ITAV, Universit\u00e9 de Toulouse, CNRS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Gallardo","sequence":"additional","affiliation":[{"name":"NeoVirTech SAS, ITAV, Universit\u00e9 de Toulouse, CNRS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4764-1708","authenticated-orcid":false,"given":"Harald","family":"Wodrich","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.12.008"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv187"},{"key":"e_1_3_3_4_2","first-page":"195","article-title":"Adenovirus","volume":"343","author":"Smith JG","year":"2010","unstructured":"Smith JG, Wiethoff CM, Stewart PL, Nemerow GR. 2010. Adenovirus. Curr Top Microbiol Immunol 343:195\u2013224.","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90382-Z"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.03.006"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.4.1992-2000.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.19.9962-9971.2002"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01428-12"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02257-14"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006217"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00581-17"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1201-1092"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02639-14"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.2.1031-1038.2001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01571-10"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00194-6"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00850-06"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2007.00618.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02540-10"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01089-17"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.08.012"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.2.794-801.2006"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.04.010"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1076"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.43.2.705-713.1982"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.7.1197"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90362-1"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90165-9"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06837.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003273"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02046-13"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12429"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006320"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.5.2421-2434.2001"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.19.5998"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.09.004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006769"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006721"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8100290"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01336-X"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137102"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.51.34511"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq783"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02545-15"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.03.078"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004187"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.08.014"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.4.1489-1496.2006"},{"key":"e_1_3_3_50_2","unstructured":"Bystricky K Gallardo F Lane D Dubarry N. 4 April 2017. US patent 09611486."},{"key":"e_1_3_3_51_2","unstructured":"Bystricky K Gallardo F Lane D Dubarry N. 27 September 2012. European patent PCT\/EP2012\/055258."},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.08.010"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.5.1654"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-7-1671"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18317"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90045-9"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07997-w"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00380-12"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003671"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.2.3.218-223.1968"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.4.3.256-263.1969"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12378"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.6.1455"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90206-3"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006455"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3930"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2680"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1137\/090774823"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1109\/ICIP.2013.6738290"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00795-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00795-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T14:38:27Z","timestamp":1646491107000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00795-18"}},"subtitle":[],"editor":[{"given":"Lawrence","family":"Banks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,9,15]]},"references-count":68,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2018,9,15]]}},"alternative-id":["10.1128\/JVI.00795-18"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00795-18","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,15]]},"assertion":[{"value":"2018-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00795-18"}}