{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:30:14Z","timestamp":1791037814253,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2009,11,15]],"date-time":"2009-11-15T00:00:00Z","timestamp":1258243200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The 2.9-A\u030a structure of the vesicular stomatitis virus nucleocapsid (N) protein bound to RNA shows the RNA to be tightly sequestered between the two lobes of the N protein. Domain movement of the lobes of the N protein has been postulated to facilitate polymerase access to the RNA template. We investigated the roles of individual amino acid residues in the C-terminal loop, involved in long-range interactions between N protein monomers, in forming functional ribonucleoprotein (RNP) templates. The effects of specific N protein mutations on its expression, interaction with the phosphoprotein, and formation of RNP templates that supported viral RNA replication and transcription were examined. Mutations introduced into the C-terminal loop, predicted to break contact with other residues in the loop, caused up to 10-fold increases in RNA replication without an equivalent stimulation of transcription. Mutation F348A, predicted to break contact between the C-terminal loop and the N-terminal arm, formed templates that supported wild-type levels of RNA replication but almost no transcription. These data show that mutations in the C-terminal loop of the N protein can disparately affect RNA replication and transcription, indicating that the N protein plays a role in modulating RNP template function beyond its structural role in RNA encapsidation.<\/jats:p>","DOI":"10.1128\/jvi.00813-09","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T00:45:59Z","timestamp":1251938759000},"page":"11429-11439","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Mutations in the C-Terminal Loop of the Nucleocapsid Protein Affect Vesicular Stomatitis Virus RNA Replication and Transcription Differentially"],"prefix":"10.1128","volume":"83","author":[{"given":"Djamila","family":"Harouaka","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Virginia, Charlottesville, Virginia"},{"name":"Department of Microbiology, University of Virginia, Charlottesville, Virginia"},{"name":"Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail W.","family":"Wertz","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Virginia, Charlottesville, Virginia"},{"name":"Department of Microbiology, University of Virginia, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.5.1504"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1125280"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.2.442"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.3.1794-1801.1997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.1.555-560.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2004.01.001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.2.1466-1475.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1130"},{"key":"e_1_3_2_10_2","first-page":"17","volume":"38","year":"1992","unstructured":"Das, T., and A. 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