{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T19:37:47Z","timestamp":1793129867478,"version":"4.5.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFE0106100"],"award-info":[{"award-number":["2024YFE0106100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Start-up Fund from Hubei Hongshan Laboratory","award":["11020107"],"award-info":[{"award-number":["11020107"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32573468"],"award-info":[{"award-number":["32573468"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2662023PY005, 2662025DKPY004"],"award-info":[{"award-number":["2662023PY005, 2662025DKPY004"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Postdoctoral Overseas Talent Introduction Program of China","award":["590323070"],"award-info":[{"award-number":["590323070"]}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Huazhong Agricultural University Scholarship","id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Shennongjia Multidisciplinary Observation and Research Station of the Ministry of Agriculture and Rural Affairs","award":["MARA-ORS070-AH013"],"award-info":[{"award-number":["MARA-ORS070-AH013"]}],"id":[{"id":"https:\/\/ror.org\/05ckt8b96","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Identifying the host factors that mediate avian influenza virus adaptation in mammals is important for monitoring zoonotic potential. Although viral polymerase adaptations are known to influence cross-species transmission, the engagement of specific host factors with divergent viral polymerases remains to be explored. We examined human DEAD-box RNA helicases (DDXs) as potential regulators of influenza polymerase activity. A screen of 16 DDXs identified DDX10 as a factor that selectively enhanced the polymerase activity and replication of human-adapted H1N1 viruses, including the 2009 pandemic strain, but not avian-origin H9N2 viruses. This differential activity was associated with DDX10 showing stronger interaction with human-origin PB1 (Cal04\/H1N1) than with avian-origin PB1 (BJ16\/H9N2). Strain specificity was mediated by two residues (336 and 364) within the PB1 catalytic domain: introducing H1N1-type residues (I336 and I364) into H9N2-PB1 conferred DDX10 responsiveness, while reciprocal mutations in H1N1-PB1 abolished it. This work defines DDX10 as a potential host factor that differentially supports influenza polymerase activity, revealing a specific molecular interface that contributes to the replication efficiency of distinct viral subtypes in human cells and providing insight into host-adaptive mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>This study identifies DDX10 as a strain-specific host factor that differentially regulates influenza A virus replication. By demonstrating that DDX10 selectively enhances human-adapted H1N1 viruses over avian-origin H9N2 viruses through direct interaction with the viral PB1 protein, and by mapping two critical residues (positions 336 and 364) in PB1 that govern this selectivity, our work provides a mechanistic framework for understanding how a single host factor can modulate viral fitness and host adaptation. These findings have important implications for predicting cross-species transmission potential and may guide the development of host-targeted antiviral strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.00815-26","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:52:54Z","timestamp":1790081574000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Interaction between DEAD-box RNA helicase 10 and influenza PB1 polymerase selectively regulates influenza A virus replication"],"prefix":"10.1128","volume":"100","author":[{"given":"Rizwan","family":"Ullah","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Hubei Hongshan 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