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Virus populations formed by quasispecies can contain resistance mutations even before a selective pressure is applied. To study the effects of the viral mutation spectrum and quasispecies, high- and low-fidelity variants have been isolated for several RNA viruses. Here, we report the discovery of a low-fidelity RdRp variant of influenza A virus that contains a substitution at Tyr82 in PB1. Viruses containing the PB1-Y82C substitution showed growth kinetics and viral RNA synthesis levels similar to those of the wild-type virus in cell culture; however, they had significantly attenuated phenotypes in a chicken egg infection experiment. These data demonstrated that decreased RdRp fidelity attenuates influenza A virus\n            <jats:italic>in vivo<\/jats:italic>\n            , which is a desirable feature for the development of safer live attenuated vaccine candidates.\n          <\/jats:p>","DOI":"10.1128\/jvi.00834-19","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T09:45:56Z","timestamp":1566812756000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Tyr82 Amino Acid Mutation in PB1 Polymerase Induces an Influenza Virus Mutator Phenotype"],"prefix":"10.1128","volume":"93","author":[{"given":"Tadasuke","family":"Naito","sequence":"first","affiliation":[{"name":"Department of Microbiology, Kawasaki Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumasa","family":"Shirai","sequence":"additional","affiliation":[{"name":"Department of Bioscience and 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