{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:11:29Z","timestamp":1789103489212,"version":"build-2803163510"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2018,8,15]],"date-time":"2018-08-15T00:00:00Z","timestamp":1534291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI122528"],"award-info":[{"award-number":["R01AI122528"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2018,8,15]]},"abstract":"<jats:p>\n                    Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma and several B-cell cancers, causing significant morbidity and mortality in immunocompromised individuals. A critical step in the production of infectious viral progeny is the packaging of the newly replicated viral DNA genome into the capsid, which involves coordination between at least seven herpesviral proteins. While the majority of these packaging factors have been well studied in related herpesviruses, the role of the KSHV ORF68 protein and its homologs remains unresolved. Here, using a KSHV mutant lacking ORF68, we confirm its requirement for viral DNA processing and packaging in infected cells. Furthermore, we show that the purified ORF68 protein directly binds DNA and is associated with a metal-dependent cleavage activity on double-stranded DNA\n                    <jats:italic>in vitro<\/jats:italic>\n                    . These activities suggest a novel role for ORF68 in herpesviral genome processing and encapsidation.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00840-18","type":"journal-article","created":{"date-parts":[[2018,6,5]],"date-time":"2018-06-05T08:52:31Z","timestamp":1528188751000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Kaposi's Sarcoma-Associated Herpesvirus ORF68 Is a DNA Binding Protein Required for Viral Genome Cleavage and Packaging"],"prefix":"10.1128","volume":"92","author":[{"given":"Matthew R.","family":"Gardner","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britt A.","family":"Glaunsinger","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, California, USA"},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, California, USA"},{"name":"Howard Hughes Medical Institute, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.7.5714-5721.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90324-R"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7030899"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-53168-7_6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.10.047"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02632-13"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061555698"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00311-13"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.5.2118-2126.2006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02648-05"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01913-14"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2007.10.016"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01509-14"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","unstructured":"Gardner MR Glaunsinger BA. 2018. Kaposi's sarcoma-associated herpesvirus ORF68 is a DNA binding protein required for viral genome cleavage and packaging. bioRxiv doi:10.1101\/322719.","DOI":"10.1101\/322719"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01967-07"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01019-12"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.03.012"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.3.1487-1506.2001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006555"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.10.5.1044-1052.1972"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-11-2879"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1421804112"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.6.3938-3946.1996"},{"key":"e_1_3_2_25_2","first-page":"460","article-title":"Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells","volume":"42","author":"Hyun HJ","year":"2001","unstructured":"Hyun HJ, Sohn JH, Ha DW, Ahn YH, Koh JY, Yoon YH. 2001. Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 42:460\u2013465.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89421-2"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20810"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02533-12"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01149"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80060-X"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01489-09"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","unstructured":"McElwee M Vijayakrishnan S Rixon F Bhella D. 2018. 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