{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:04:48Z","timestamp":1790229888460,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["20wm0325005h"],"award-info":[{"award-number":["20wm0325005h"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Science, Sports and Technology of Japan","doi-asserted-by":"crossref","award":["19H05286"],"award-info":[{"award-number":["19H05286"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["22gm6410022h"],"award-info":[{"award-number":["22gm6410022h"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["Grants for Scientific Research"],"award-info":[{"award-number":["Grants for Scientific Research"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Science, Sports and Technology of Japan","doi-asserted-by":"crossref","award":["LEADER"],"award-info":[{"award-number":["LEADER"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"MSD Life Science Foundation","award":["Research grant"],"award-info":[{"award-number":["Research grant"]}]},{"DOI":"10.13039\/100031489","name":"Shionogi Infectious Disease Research Promotion Foundation","doi-asserted-by":"crossref","award":["Research grant"],"award-info":[{"award-number":["Research grant"]}],"id":[{"id":"10.13039\/100031489","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Chemo-Sero-Therapeutic Research Institute","award":["Research grant"],"award-info":[{"award-number":["Research grant"]}]},{"name":"The Waksman Foundation of Japan","award":["Research grant"],"award-info":[{"award-number":["Research grant"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2025,9,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Herpesviruses replicate their genomes and package them into capsids within the host cell nucleus. These capsids must then translocate from the nucleus to the cytoplasm through a process designated nuclear egress. The virus-encoded nuclear egress complex (NEC), consisting of a nuclear matrix protein and a nuclear membrane protein, plays a crucial role in this process. Although the role of NEC for nucleo-cytoplasmic transport of capsids is conserved in\n                      <jats:italic toggle=\"yes\">Herpesviridae<\/jats:italic>\n                      , some of the binding partners of the NEC components are specific for individual viruses. The NECs of alpha- and gammaherpesviruses recruit the Endosomal Sorting Complex Required for Transport III (ESCRT-III) to the inner nuclear membrane for efficient nuclear egress of capsids. In contrast, the role of ESCRT-III for nuclear egress of betaherpesviruses, including human cytomegalovirus (HCMV) and human herpesvirus 6A (HHV-6A), has not been elucidated. Here, we show that ESCRT-III is recruited to the nuclear rim in cells expressing the NEC of HCMV or of HHV-6A. Inhibition of ESCRT-III impaired HHV-6A replication and nuclear egress of the capsids. Mechanistically, ESCRT-III adaptor ALIX interacts with HHV-6A NEC membrane protein U34 and thus contributes to HHV-6A replication. From these observations, we conclude that, like at least some alpha- and gammaherpesviruses, HHV-6A NEC recruits ESCRT-III through ALIX to promote viral capsid nuclear egress.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        ESCRT-III performs reverse-topology scission involved in many diverse cellular processes, including cytokinesis, endosome maturation, autophagy, membrane repair, and viral budding. Nucleo-cytoplasmic transport of herpesvirus capsids requires scission at the inner nuclear membrane. In alpha- and gammaherpesviruses, this process requires ESCRT-III, but it is not known whether this is also the case for betaherpesviruses. Here, we show that ESCRT-III is also important for nuclear egress of capsids of the betaherpesvirus human herpesvirus 6A. These results imply that ESCRT-III-mediated inner nuclear membrane scission is a conserved feature in the virion maturation process of\n                        <jats:italic toggle=\"yes\">Herpesviridae<\/jats:italic>\n                        . Our findings thus suggest that ESCRT-III is a potential therapeutic target also for betaherpesvirus infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.00844-25","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T13:03:02Z","timestamp":1755608582000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["ESCRT-III is recruited by human herpesvirus 6A nuclear egress complex to promote nuclear egress of the nucleocapsid"],"prefix":"10.1128","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6616-1192","authenticated-orcid":false,"given":"Aila","family":"Gulijiahani","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tgsfw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine","place":["Kobe, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1689-4016","authenticated-orcid":true,"given":"Jun","family":"Arii","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tgsfw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine","place":["Kobe, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vuk","family":"Isakovic","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tgsfw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine","place":["Kobe, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing Rin","family":"Huang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tgsfw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine","place":["Kobe, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8232-6925","authenticated-orcid":true,"given":"Yasushi","family":"Kawaguchi","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo","place":["Minato-ku, Japan"]},{"name":"Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo","place":["Minato-ku, Japan"]},{"name":"Research Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo","place":["Minato-ku, Japan"]},{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"name":"Pandemic Preparedness, Infection and Advanced Research Center, The University of Tokyo","place":["Minato-ku, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6595-8522","authenticated-orcid":true,"given":"Yasuko","family":"Mori","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tgsfw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine","place":["Kobe, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2559"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13050754"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells11111837"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.75.18.8803-8817.2001"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.07.021"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82924-0"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.11.018"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00718-23"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.74.1.117-129.2000"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701757104"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5131"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01290-19"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201592359"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1511140112"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201592651"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.11.008"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.011546"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010623"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C115.686527"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-072909-101058"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0177-4"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00222-07"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00574-09"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02777-14"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002904"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05889-9"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01235-16"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01704-21"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02096-17"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012300"},{"key":"e_1_3_5_32_2","first-page":"2058","volume-title":"Fields Virology","author":"Yamanishi K MY","year":"2013","unstructured":"Yamanishi KMY, PelletPE. 2013. Human herpesviruses 6 and 7, p 2058\u20132079. In Fields Virology. Wolters Kluwer Health."},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002951"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(06)70007-5"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12030303"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75852-6"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(01)00506-2"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.11.2982"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143422"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601850"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005938107"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01093-09"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00796.x"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.08.018"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00714-1"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801567105"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb400495h"},{"key":"e_1_3_5_48_2","first-page":"1802","volume-title":"Fields virology","author":"Pellet PE RB","year":"2013","unstructured":"Pellet PE RB. 2013. Herpesviridae, p 1802\u20131822. In Fields virology, 6th ed. Lippincott-Williams & Wilkins, Philadelphia, PA.","edition":"6"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.3389\/fviro.2024.1378054"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.08.012"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.08.029"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.11.008"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.09.044"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00190-21"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02466-20"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01396-16"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01269-21"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01264-22"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1242\/bio.055343"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.2144\/000113909"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.035"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.4.2452-2458.2003"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.20.13037-13046.2005"},{"key":"e_1_3_5_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01572-20"},{"key":"e_1_3_5_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00498-19"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00844-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00844-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T21:52:53Z","timestamp":1758664373000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.00844-25"}},"subtitle":[],"editor":[{"given":"Anna Ruth","family":"Cliffe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,23]]},"references-count":64,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,23]]}},"alternative-id":["10.1128\/jvi.00844-25"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00844-25","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,23]]},"assertion":[{"value":"2025-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00844-25"}}