{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:16:23Z","timestamp":1792588583395,"version":"4.5.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Western equine encephalitis virus (WEEV;\n            <jats:italic>Togaviridae<\/jats:italic>\n            ,\n            <jats:italic>Alphavirus<\/jats:italic>\n            ) is an enveloped RNA virus that is typically transmitted to vertebrate hosts by infected mosquitoes. WEEV is an important cause of viral encephalitis in humans and horses in the Americas, and infection results in a range of disease, from mild flu-like illnesses to encephalitis, coma, and death. In addition to spreading via mosquito vectors, human WEEV infections can potentially occur directly via aerosol transmission. Due to its aerosol infectivity and virulence, WEEV is thus classified as a biological safety level 3 (BSL-3) agent. Because of its highly infectious nature and containment requirements, it has not been possible to investigate WEEV's structure or assembly mechanism using standard structural biology techniques. Thus, to image WEEV and other BSL-3 agents, we have constructed a first-of-its-kind BSL-3 cryoelectron microscopy (cryoEM) containment facility. cryoEM images of WEEV were used to determine the first three-dimensional structure of this important human pathogen. The overall organization of WEEV is similar to those of other alphaviruses, consistent with the high sequence similarity among alphavirus structural proteins. Surprisingly, the nucleocapsid of WEEV, a New World virus, is more similar to the Old World alphavirus Sindbis virus than to other New World alphaviruses.\n          <\/jats:p>","DOI":"10.1128\/jvi.00876-10","type":"journal-article","created":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T02:47:24Z","timestamp":1279162044000},"page":"9775-9782","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Structure of the Recombinant Alphavirus Western Equine Encephalitis Virus Revealed by Cryoelectron Microscopy"],"prefix":"10.1128","volume":"84","author":[{"given":"Michael B.","family":"Sherman","sequence":"first","affiliation":[{"name":"W. M. Keck Center for Virus Imaging"},{"name":"Sealy Center for Structural Biology and Biophysics"},{"name":"Department of Biochemistry and Molecular Biology"},{"name":"Institute for Human Infections and Immunity"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott C.","family":"Weaver","sequence":"additional","affiliation":[{"name":"W. M. Keck Center for Virus Imaging"},{"name":"Sealy Center for Structural Biology and Biophysics"},{"name":"Institute for Human Infections and Immunity"},{"name":"Center for Biodefense and Emerging Infectious Diseases"},{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0020"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.23.12250-12258.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90516-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01191-08"},{"key":"e_1_3_2_6_2","first-page":"1023","year":"2007","unstructured":"Griffin, D. E. 2007. Alphaviruses, p. 1023-1068. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 5th ed. 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