{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:49:21Z","timestamp":1791413361998,"version":"4.3.3"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2013,9,15]],"date-time":"2013-09-15T00:00:00Z","timestamp":1379203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The interferon-inducible protein with tetratricopeptide (IFIT) family proteins inhibit replication of some viruses by recognizing several types of RNAs, including 5\u2032-triphosphate RNA and 5\u2032 capped 2\u2032-O unmethylated mRNA. However, it remains unclear how IFITs inhibit replication of some viruses through recognition of RNA. Here, we analyzed the mechanisms by which Ifit1 exerts antiviral responses. Replication of a Japanese encephalitis virus (JEV) 2\u2032-O methyltransferase (MTase) mutant was markedly enhanced in mouse embryonic fibroblasts and macrophages lacking Ifit1. Ifit1 bound 5\u2032-triphosphate RNA but more preferentially associated with 5\u2032 capped 2\u2032-O unmethylated mRNA. Ifit1 inhibited the translation of mRNA and thereby restricted the replication of JEV mutated in 2\u2032-O MTase. Thus, Ifit1 inhibits replication of MTase-defective JEV by inhibiting mRNA translation through direct binding to mRNA 5\u2032 structures.<\/jats:p>","DOI":"10.1128\/jvi.00883-13","type":"journal-article","created":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T08:20:19Z","timestamp":1372926019000},"page":"9997-10003","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Ifit1 Inhibits Japanese Encephalitis Virus Replication through Binding to 5\u2032 Capped 2\u2032-O Unmethylated RNA"],"prefix":"10.1128","volume":"87","author":[{"given":"Taishi","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Laboratory of Mucosal Immunology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Katoh","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisako","family":"Kayama","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Laboratory of Mucosal Immunology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Saiga","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Okuyama","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Umemoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Laboratory of Mucosal Immunology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Laboratory of Mucosal Immunology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan"},{"name":"Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/255033a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/266235a0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.155283"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr038"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90009-4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90091-4"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(81)90176-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00814-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00407-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001038"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02704-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2008.05.003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09489"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1979"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002698"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/261611a0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-74-3-485"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15623"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00716.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2010.0101"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.130"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3344"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406700200"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.06.011"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605771200"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01593-08"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900818106"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100963"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607830103"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.241"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.111"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2048"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11783"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.06.009"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301206"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.6.3448-3458.2005"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80638-0"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00846-11"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02806-12"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.12.015"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.44.2.175-205.1980"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.046797-0"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.7.3898-3912.2003"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002712"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00883-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00883-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:56:56Z","timestamp":1646488616000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00883-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,15]]},"references-count":44,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2013,9,15]]}},"alternative-id":["10.1128\/JVI.00883-13"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00883-13","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,15]]},"assertion":[{"value":"2013-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}