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To facilitate such studies, we utilized a predictive algorithm to identify candidate HLA-A2 supertype epitopes from the LASV nucleoprotein and glycoprotein precursor (GPC) genes. We identified three peptides (GPC<jats:sub>42-50<\/jats:sub>, GLVGLVTFL; GPC<jats:sub>60-68<\/jats:sub>, SLYKGVYEL; and GPC<jats:sub>441-449<\/jats:sub>, YLISIFLHL) that displayed high-affinity binding (\u226498 nM) to HLA-A*0201, induced CD8<jats:sup>+<\/jats:sup>T-cell responses of high functional avidity in HLA-A*0201 transgenic mice, and were naturally processed from native LASV GPC in human HLA-A*0201-positive target cells. HLA-A*0201 mice immunized with either GPC<jats:sub>42-50<\/jats:sub>or GPC<jats:sub>60-68<\/jats:sub>were protected against challenge with a recombinant vaccinia virus that expressed LASV GPC. The epitopes identified in this study represent potential diagnostic reagents and candidates for inclusion in epitope-based vaccine constructs. Our approach is applicable to any pathogen with existing sequence data, does not require manipulation of the actual pathogen or access to immune human donors, and should therefore be generally applicable to category A through C agents and other emerging pathogens.<\/jats:p>","DOI":"10.1128\/jvi.00896-06","type":"journal-article","created":{"date-parts":[[2006,8,15]],"date-time":"2006-08-15T22:42:56Z","timestamp":1155681776000},"page":"8351-8361","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Identification of Protective Lassa Virus Epitopes That Are Restricted by HLA-A2"],"prefix":"10.1128","volume":"80","author":[{"given":"Jason","family":"Botten","sequence":"first","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California, 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeff","family":"Alexander","sequence":"additional","affiliation":[{"name":"Pharmexa-Epimmune, San Diego, California, 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Pasquetto","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, San Diego, California, 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Sidney","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, San Diego, California, 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polly","family":"Barrowman","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California, 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joey","family":"Ting","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California, 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjoern","family":"Peters","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, San Diego, California, 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Southwood","sequence":"additional","affiliation":[{"name":"Pharmexa-Epimmune, San Diego, California, 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Stewart","sequence":"additional","affiliation":[{"name":"Pharmexa-Epimmune, San Diego, California, 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria P.","family":"Rodriguez-Carreno","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California, 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Mothe","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, San Diego, California, 92121"},{"name":"Department of Biological Sciences, California State University, San Marcos, California 92096"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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