{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:53:38Z","timestamp":1791312818974,"version":"4.3.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Three distinct chimpanzee Fabs against the A33 envelope glycoprotein of vaccinia virus were isolated and converted into complete monoclonal antibodies (MAbs) with human \u03b31 heavy-chain constant regions. The three MAbs (6C, 12C, and 12F) displayed high binding affinities to A33 (\n                    <jats:italic>\n                      K\n                      <jats:sub>d<\/jats:sub>\n                    <\/jats:italic>\n                    of 0.14 nM to 20 nM) and may recognize the same epitope, which was determined to be conformational and located within amino acid residues 99 to 185 at the C terminus of A33. One or more of the MAbs were shown to reduce the spread of vaccinia virus as well as variola virus (the causative agent of smallpox) in vitro and to more effectively protect mice when administered before or 2 days after intranasal challenge with virulent vaccinia virus than a previously isolated mouse anti-A33 MAb (1G10) or vaccinia virus immunoglobulin. The protective efficacy afforded by anti-A33 MAb was comparable to that of a previously isolated chimpanzee\/human anti-B5 MAb. The combination of anti-A33 MAb and anti-B5 MAb did not synergize the protective efficacy. These chimpanzee\/human anti-A33 MAbs may be useful in the prevention and treatment of vaccinia virus-induced complications of vaccination against smallpox and may also be effective in the immunoprophylaxis and immunotherapy of smallpox and other orthopoxvirus diseases.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00906-07","type":"journal-article","created":{"date-parts":[[2007,6,20]],"date-time":"2007-06-20T21:50:37Z","timestamp":1182376237000},"page":"8989-8995","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Characterization of Chimpanzee\/Human Monoclonal Antibodies to Vaccinia Virus A33 Glycoprotein and Its Variola Virus Homolog In Vitro and in a Vaccinia Virus Mouse Protection Model"],"prefix":"10.1128","volume":"81","author":[{"given":"Zhaochun","family":"Chen","sequence":"first","affiliation":[{"name":"Hepatitis Viruses Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Earl","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Americo","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger","family":"Damon","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott K.","family":"Smith","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fujuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Hepatitis Viruses Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Sebrell","sequence":"additional","affiliation":[{"name":"Hepatitis Viruses Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Emerson","sequence":"additional","affiliation":[{"name":"Molecular Hepatitis Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roselyn J.","family":"Eisenberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna","family":"Gorshkova","sequence":"additional","affiliation":[{"name":"Protein Biophysics Resource, National Institute for Biomedical Imaging and Bioengineering, NIH, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Schuck","sequence":"additional","affiliation":[{"name":"Protein Biophysics Resource, National Institute for Biomedical Imaging and Bioengineering, NIH, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Satterfield","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Texas, M. 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