{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:34:40Z","timestamp":1792809280967,"version":"4.5.0"},"reference-count":73,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An interaction between the helicase domain of the\n            <jats:italic>Tobacco mosaic virus<\/jats:italic>\n            (TMV) 126-\/183-kDa replicase protein(s) and the\n            <jats:italic>Arabidopsis thaliana<\/jats:italic>\n            NAC domain transcription factor ATAF2 was identified via yeast two-hybrid and in planta immunoprecipitation assays. ATAF2 is transcriptionally induced in response to TMV infection, and its overexpression significantly reduces virus accumulation. Proteasome inhibition studies suggest that ATAF2 is targeted for degradation during virus infection. The transcriptional activity of known defense-associated marker genes\n            <jats:italic>PR1<\/jats:italic>\n            ,\n            <jats:italic>PR2<\/jats:italic>\n            , and\n            <jats:italic>PDF1.2<\/jats:italic>\n            significantly increase within transgenic plants overexpressing ATAF2. In contrast, these marker genes have reduced transcript levels in ATAF2 knockout or repressor plant lines. Thus, ATAF2 appears to function in the regulation of host basal defense responses. In response to TMV infections,\n            <jats:italic>ATAF2<\/jats:italic>\n            and\n            <jats:italic>PR1<\/jats:italic>\n            display increased transcript accumulations in inoculated tissues but not in systemically infected tissues.\n            <jats:italic>ATAF2<\/jats:italic>\n            and\n            <jats:italic>PR1<\/jats:italic>\n            transcript levels also increase in response to salicylic acid treatment. However, the salicylic acid treatment of systemically infected tissues did not produce a similar increase in either\n            <jats:italic>ATAF2<\/jats:italic>\n            or\n            <jats:italic>PR1<\/jats:italic>\n            transcripts, suggesting that host defense responses are attenuated during systemic virus invasion. Similarly, noninfected ATAF2 knockout or ATAF2 repressor lines display reduced levels of\n            <jats:italic>PR1<\/jats:italic>\n            transcripts when treated with salicylic acid. Taken together, these findings suggest that the replicase-ATAF2 interaction suppresses basal host defenses as a means to promote systemic virus accumulation.\n          <\/jats:p>","DOI":"10.1128\/jvi.00941-09","type":"journal-article","created":{"date-parts":[[2009,7,23]],"date-time":"2009-07-23T01:09:51Z","timestamp":1248311391000},"page":"9720-9730","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Interaction of the\n            <i>Tobacco Mosaic Virus<\/i>\n            Replicase Protein with a NAC Domain Transcription Factor Is Associated with the Suppression of Systemic Host Defenses"],"prefix":"10.1128","volume":"83","author":[{"given":"Xiao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameer 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