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The CD8<jats:sup>+<\/jats:sup>T cell response to a virus is restricted to several peptides, and the magnitudes of the effector as well as memory phases of the response to the individual peptides are generally hierarchical. The peptide eliciting a stronger response is called immunodominant (ID), and those with smaller-magnitude responses are termed subdominant (SD). The relative importance of ID and SD determinants in protective immunity remains to be fully elucidated. We previously showed that multispecific memory CD8<jats:sup>+<\/jats:sup>T cells can protect susceptible mice from mousepox, an acute lethal viral disease. It remained unknown, however, whether CD8<jats:sup>+<\/jats:sup>T cells specific for single ID or SD peptides could be protective. Here, we demonstrate that immunization with dendritic cells pulsed with ID and some but not all SD peptides induces memory CD8<jats:sup>+<\/jats:sup>T cells that are fully capable of protecting susceptible mice from mousepox. Additionally, while natural killer (NK) cells are essential for the natural resistance of nonimmune C57BL\/6 (B6) to mousepox, we show that memory CD8<jats:sup>+<\/jats:sup>T cells of single specificity also protect B6 mice depleted of NK cells. This suggests it is feasible to produce effective antiviral CD8<jats:sup>+<\/jats:sup>T cell vaccines using single CD8<jats:sup>+<\/jats:sup>T cell determinants and that NK cells are no longer essential when memory CD8<jats:sup>+<\/jats:sup>T cells are present.<\/jats:p>","DOI":"10.1128\/jvi.00981-12","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T03:47:43Z","timestamp":1340855263000},"page":"9748-9759","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Memory CD8<sup>+<\/sup>T Cells Specific for a Single Immunodominant or Subdominant Determinant Induced by Peptide-Dendritic Cell Immunization Protect from an Acute Lethal Viral Disease"],"prefix":"10.1128","volume":"86","author":[{"given":"Sanda","family":"Remakus","sequence":"first","affiliation":[{"name":"Fox Chase Cancer Center, Immune Cell Development and Host Defense Program, Philadelphia, Pennsylvania, USA"},{"name":"Jefferson Medical College of Thomas Jefferson University, Department of Microbiology and Immunology, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Rubio","sequence":"additional","affiliation":[{"name":"Fox Chase Cancer Center, Immune Cell Development and Host Defense Program, Philadelphia, Pennsylvania, USA"},{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas and Universidad Aut\u00f3noma de Madrid, Campus de Cantoblanco, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueying","family":"Ma","sequence":"additional","affiliation":[{"name":"Fox Chase Cancer Center, Immune Cell Development and Host Defense Program, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis J.","family":"Sigal","sequence":"additional","affiliation":[{"name":"Fox Chase Cancer Center, Immune Cell Development and Host Defense Program, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"5","article-title":"Antigen presentation by B lymphocytes: mechanisms and functional significance","volume":"1","author":"Abbas AK","year":"1989","unstructured":"AbbasAK. 1989. 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