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Flaviviruses have expanded into new populations and emerged as novel pathogens repeatedly in recent years, demonstrating that they remain a global threat. Currently, there are no approved antiviral therapeutics against either Kunjin or West Nile viruses. Thus, there is a pressing need for understanding the pathogenesis of these viruses in humans. In this study, we investigated the role of the Kunjin virus helicase on infection in cell culture and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . This work provides new insight into how flaviviruses control pathogenesis and mosquito transmission through the nonstructural protein 3 helicase.\n                  <\/jats:p>","DOI":"10.1128\/jvi.01021-20","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T09:33:02Z","timestamp":1595496782000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["A Hyperactive Kunjin Virus NS3 Helicase Mutant Demonstrates Increased Dissemination and Mortality in Mosquitoes"],"prefix":"10.1128","volume":"94","author":[{"given":"Kelly E.","family":"Du Pont","sequence":"first","affiliation":[{"name":"Department of Chemistry, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole R.","family":"Sexton","sequence":"additional","affiliation":[{"name":"Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"},{"name":"Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"McCullagh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory D.","family":"Ebel","sequence":"additional","affiliation":[{"name":"Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"},{"name":"Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8180-5772","authenticated-orcid":false,"given":"Brian J.","family":"Geiss","sequence":"additional","affiliation":[{"name":"Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"},{"name":"Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA"},{"name":"School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1805.111720"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.11-0165"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra030476"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.10.008"},{"key":"e_1_3_2_6_2","volume-title":"West Nile virus key facts","author":"World Health Organization","year":"2017","unstructured":"World Health Organization. 2017. 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