{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:09:01Z","timestamp":1790917741360,"version":"4.1.0"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chikungunya virus (CHIKV) is a reemerging mosquito-transmitted alphavirus that causes epidemics of debilitating polyarthritis in humans. A prior study identified two anti-CHIKV monoclonal antibodies ([MAbs] CHK-152 and CHK-166) against the E2 and E1 structural proteins, which had therapeutic efficacy in immunocompetent and immunocompromised mice. Combination MAb therapy was required as administration of a single MAb resulted in the rapid selection of neutralization escape variants and treatment failure in mice. Here, we initially evaluated the efficacy of combination MAb therapy in a nonhuman primate model of CHIKV infection. Treatment of rhesus macaques with CHK-152 and CHK-166 reduced viral spread and infection in distant tissue sites and also neutralized reservoirs of infectious virus. Escape viruses were not detected in the residual viral RNA present in tissues and organs of rhesus macaques. To evaluate the possible significance of MAb resistance, we engineered neutralization escape variant viruses (E1-K61T, E2-D59N, and the double mutant E1-K61T E2-D59N) that conferred resistance to CHK-152 and CHK-166 and tested them for fitness in mosquito cells, mammalian cells, mice, and\n                    <jats:named-content content-type=\"genus-species\">Aedes albopictus<\/jats:named-content>\n                    mosquitoes. In both cell culture and mosquitoes, the mutant viruses grew equivalently and did not revert to wild-type (WT) sequence. All escape variants showed evidence of mild clinical attenuation, with decreased musculoskeletal disease at early times after infection in WT mice and a prolonged survival time in immunocompromised\n                    <jats:italic>Ifnar1<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. Unexpectedly, this was not associated with decreased infectivity, and consensus sequencing from tissues revealed no evidence of reversion or compensatory mutations. Competition studies with CHIKV WT also revealed no fitness compromise of the double mutant (E1-K61T E2-D59N) neutralization escape variant in WT mice. Collectively, our study suggests that neutralization escape viruses selected during combination MAb therapy with CHK-152 plus CHK-166 retain fitness, cause less severe clinical disease, and likely would not be purified during the enzootic cycle.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Chikungunya virus (CHIKV) causes explosive epidemics of acute and chronic arthritis in humans in Africa, the Indian subcontinent, and Southeast Asia and recently has spread to the New World. As there are no approved vaccines or therapies for human use, the possibility of CHIKV-induced debilitating disease is high in many parts of the world. To this end, our laboratory recently generated a combination monoclonal antibody therapy that aborted lethal and arthritogenic disease in wild-type and immunocompromised mice when administered as a single dose several days after infection. In this study, we show the efficacy of the antibody combination in nonhuman primates and also evaluate the significance of possible neutralization escape mutations in mosquito and mammalian cells, mice, and\n                    <jats:named-content content-type=\"genus-species\">Aedes albopictus<\/jats:named-content>\n                    vector mosquitoes. Our experiments show that escape viruses from combination antibody therapy cause less severe CHIKV clinical disease, retain fitness, and likely would not be purified by mosquito vectors.\n                  <\/jats:p>","DOI":"10.1128\/jvi.01032-14","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T21:56:52Z","timestamp":1400104612000},"page":"8213-8226","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Chikungunya Viruses That Escape Monoclonal Antibody Therapy Are Clinically Attenuated, Stable, and Not Purified in Mosquitoes"],"prefix":"10.1128","volume":"88","author":[{"given":"Pankaj","family":"Pal","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie M.","family":"Fox","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Hawman","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Jang S.","family":"Huang","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute and Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Messaoudi","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"},{"name":"Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, USA"},{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon, USA"},{"name":"University of California\u2014Riverside, Riverside, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Kreklywich","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Denton","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred W.","family":"Legasse","sequence":"additional","affiliation":[{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia P.","family":"Smith","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syd","family":"Johnson","sequence":"additional","affiliation":[{"name":"MacroGenics, Rockville, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Axthelm","sequence":"additional","affiliation":[{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana L.","family":"Vanlandingham","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute and Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Streblow","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"},{"name":"Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, USA"},{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Higgs","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute and Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Morrison","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, University of Colorado School of Medicine, Aurora, Colorado, 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Chikungunya in north-eastern Italy: a summing up of the outbreak. Euro Surveill. 12:E0711220.2. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=3313.","journal-title":"Euro Surveill."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61779-6"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2013.07.006"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.07.024"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.84"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030201"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2006.0562"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp078013"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.64"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60336-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.92"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2013.03.003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002137"},{"key":"e_1_3_3_20_2","first-page":"471","article-title":"Re-emergence of Chikungunya and O'Nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships","volume":"81","author":"Powers AM","year":"2000","unstructured":"PowersAMBraultACTeshRBWeaverSC. 2000. 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