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In this work, we have identified the first case of a swine-origin influenza A(H1N1)pdm09 virus resulting in a human infection. This shows that these viruses not only circulate in swine hosts, but are continuing to evolve and distinguish themselves from previously circulating human-origin influenza viruses. The development of techniques for distinguishing human-origin and swine-origin viruses are necessary for the continued surveillance of influenza viruses. We show that unique genetic signatures can differentiate circulating swine-associated strains from circulating human-associated strains of influenza A(H1N1)pdm09, and these signatures can be used to enhance surveillance of swine-origin influenza.<\/jats:p>","DOI":"10.1128\/jvi.01066-20","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T14:12:48Z","timestamp":1603980768000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Detection and Characterization of Swine Origin Influenza A(H1N1) Pandemic 2009 Viruses in Humans following Zoonotic Transmission"],"prefix":"10.1128","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2058-5646","authenticated-orcid":false,"given":"Peter W.","family":"Cook","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, Influenza Division, Atlanta, Georgia, USA"},{"name":"Association of Public 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