{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:57:05Z","timestamp":1790906225864,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Varicella-zoster virus (VZV) is the first of the human herpesviruses to be attenuated and subsequently approved as a live vaccine to prevent varicella and herpes zoster. Both the attenuated VZV vaccine, called vaccine Oka or vOka, and the parental strain pOka have been completely sequenced. Yet the specific determinants of attenuation are uncertain. The open reading frame (ORF) with the most single nucleotide polymorphisms (SNPs), ORF62, encodes the regulatory protein IE62, but IE62 studies have failed to define a specific SNP associated with attenuation. We have completed next-generation sequencing of the VZV Ellen genome, a strain known to be highly attenuated by its very limited replication in human skin xenografts in the SCID mouse model of VZV pathogenesis. A comparative analysis of the Ellen sequence with all other complete VZV sequences was extremely informative. In particular, an unexpected finding was a stop codon mutation in Ellen ORF0 (herpes simplex virus UL56 homolog) identical to one found in vOka, combined with the absence of polymorphisms in most Ellen ORFs that were known to be mutated in vOka. The mutated ORF0 protein was also imaged in both two dimensions and three dimensions by confocal microscopy. The probability of two VZV strains not connected by a recent common ancestor having an identical ORF0 SNP by chance would be 1 \u00d7 10\n            <jats:sup>\u22128<\/jats:sup>\n            , in other words, extremely unlikely. Taken together, these bioinformatics analyses strongly suggest that the stop codon ORF0 SNP is one of the determinants of the attenuation genotype of live VZV vaccines.\n          <\/jats:p>","DOI":"10.1128\/jvi.01067-12","type":"journal-article","created":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T07:52:34Z","timestamp":1343375554000},"page":"10695-10703","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["The Attenuated Genotype of Varicella-Zoster Virus Includes an ORF0 Transitional Stop Codon Mutation"],"prefix":"10.1128","volume":"86","author":[{"given":"Geoffrey A.","family":"Peters","sequence":"first","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun D.","family":"Tyler","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Carpenter","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Children's Hospital, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallen","family":"Jackson","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Children's Hospital, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann M.","family":"Arvin","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Microbiology and Immunology, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Grose","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Children's Hospital, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"75","article-title":"Investigations of the pathogenesis of varicella zoster virus infection in the SCIDhu mouse model","volume":"13","author":"Arvin AM","year":"2006","unstructured":"ArvinAM. 2006. Investigations of the pathogenesis of varicella zoster virus infection in the SCIDhu mouse model. Herpes 13:75\u201380.","journal-title":"Herpes"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.017814-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2307\/4592257"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01821-07"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.5.2076-2082.2006"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-9-1759"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08870.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2008.03.021"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1359"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0775"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/522165"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.22.11447-11459.2002"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.000423"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00357-12"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.13.6799-6807.2004"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00878-10"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.23.11311-11321.2000"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-547"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.13.6718-6728.2002"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.06.016"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.965-974.1998"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.3.1987-1992.1994"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.3.1350-1359.1994"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051016"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.8.4474-4483.1993"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00715-06"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.2144\/000112511"},{"key":"e_1_3_3_29_2","first-page":"43","article-title":"Roles of cellular transcription factors in VZV replication","volume":"342","author":"Ruyechan WT","year":"2010","unstructured":"RuyechanWT. 2010. Roles of cellular transcription factors in VZV replication. Curr. Top. Microbiol. Immunol. 342:43\u201365.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.10.5607-5620.2003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1156"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.09.031"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00668-06"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.4.2.199"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.09.037"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198312083092306"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01722-07"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06233-11"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.10.047"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01067-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01067-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:18:14Z","timestamp":1646493494000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01067-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":38,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1128\/JVI.01067-12"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01067-12","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10]]},"assertion":[{"value":"2012-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-07-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}