{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:07:49Z","timestamp":1791047269547,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The transmission of variant Creutzfeldt-Jakob disease (vCJD) through blood transfusions has created new concerns about the iatrogenic spread of transmissible spongiform encephalopathies (TSEs)\/prion diseases through blood and plasma-derived products and has increased the need to develop efficient methods for detection of the agent in biologics. Here, we report the first successful generation of spleen-derived murine stromal cell cultures that persistently propagate two mouse-adapted isolates of human TSE agents, mouse-adapted vCJD, and Fukuoka 1. These new cell cultures can be used efficiently for studies of the pathogenesis of the disease, for development of diagnostics and therapeutics, and as a rapid ex vivo assay for TSE inactivation\/removal procedures.<\/jats:p>","DOI":"10.1128\/jvi.01085-08","type":"journal-article","created":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T00:15:13Z","timestamp":1219277713000},"page":"10959-10962","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Persistent Propagation of Variant Creutzfeldt-Jakob Disease Agent in Murine Spleen Stromal Cell Culture with Features of Mesenchymal Stem Cells"],"prefix":"10.1128","volume":"82","author":[{"given":"Sergey","family":"Akimov","sequence":"first","affiliation":[{"name":"Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oksana","family":"Yakovleva","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Vasilyeva","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carroll","family":"McKenzie","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larisa","family":"Cervenakova","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.11.7104-7112.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400158101"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05411-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.62.5.1558-1564.1988"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0041-1132.2003.00586.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1205.051348"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/225100a0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/261144a0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83344-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02628-06"},{"key":"e_1_3_2_12_2","first-page":"E070118.4","volume":"12","year":"2007","unstructured":"Eurosurveillance editorial office. 18 January 2007. 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