{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:33:59Z","timestamp":1787016839004,"version":"build-2736575974"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T00:00:00Z","timestamp":1606089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201400006C"],"award-info":[{"award-number":["HHSN272201400006C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012524","name":"American Lebanese Syrian Associated Charities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012524","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,11,23]]},"abstract":"<jats:p>\n                    Robust\n                    <jats:italic>in vitro<\/jats:italic>\n                    culture systems for influenza virus are critically needed. MDCK cells, the most widely used cell line for influenza isolation and propagation, do not adequately model the respiratory tract. Therefore, many clinical isolates, both animal and human, are unable to be isolated and characterized, limiting our understanding of currently circulating influenza viruses. We have developed immortalized swine respiratory epithelial cells that retain the ability to differentiate and can support influenza replication and isolation. These cell lines can be used as additional tools to enhance influenza research and vaccine development.\n                  <\/jats:p>","DOI":"10.1128\/jvi.01091-20","type":"journal-article","created":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T09:51:35Z","timestamp":1600768295000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and Propagation of Influenza A Viruses"],"prefix":"10.1128","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-9177","authenticated-orcid":false,"given":"Victoria","family":"Meliopoulos","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Cherry","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Wohlgemuth","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah","family":"Honce","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, USA"},{"name":"Integrated Program in Biomedical Sciences, College of Graduate Health Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6283-4543","authenticated-orcid":false,"given":"Karen","family":"Barnard","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2540-8769","authenticated-orcid":false,"given":"Phillip","family":"Gauger","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Davis","sequence":"additional","affiliation":[{"name":"Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Shult","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1836-6655","authenticated-orcid":false,"given":"Colin","family":"Parrish","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-727X","authenticated-orcid":false,"given":"Stacey","family":"Schultz-Cherry","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6722a4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.7189\/jogh.09.020421"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01077-14"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3791\/52421:e52421"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05582-11"},{"key":"e_1_3_2_7_2","first-page":"39","article-title":"Growth and immunogenicity of influenza viruses cultivated in Vero or MDCK cells and in embryonated chicken eggs","volume":"98","author":"Govorkova EA","year":"1999","unstructured":"Govorkova EA, Kodihalli S, Alymova IV, Fanget B, Webster RG. 1999. Growth and immunogenicity of influenza viruses cultivated in Vero or MDCK cells and in embryonated chicken eggs. Dev Biol Stand 98:39\u201351.","journal-title":"Dev Biol Stand"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.9.2.175-179.1979"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00398-08"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.16.4.588-594.1968"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.15.8418-8425.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4139-4146.2005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0609-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308001101"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2009.131082"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2012.33"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2601.191144"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2012.01508.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6542a1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2014.03.022"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2012.00333.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.115998"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-135"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00642-10"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.000176"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02677-05"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021183"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.19.9517-9525.2001"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep39668"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gln073"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(61)90192-6"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38339"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00399.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-015-1351-1"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12671"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.031203-0"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-9-2183"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1404"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00841-14"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01349-14"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2018.08.012"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.4172\/1747-0862.1000028"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-016-0719-8"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.33354"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002134"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/440435a"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090949"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1125548"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(93)90056-s"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03005-12"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008713"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03575-13"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.08.006"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines6020028"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712377114"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1461"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.16777"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03248-15"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3479"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01091-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01091-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:01:29Z","timestamp":1646474489000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01091-20"}},"subtitle":[],"editor":[{"given":"Rebecca Ellis","family":"Dutch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,23]]},"references-count":59,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2020,11,23]]}},"alternative-id":["10.1128\/JVI.01091-20"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01091-20","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,23]]},"assertion":[{"value":"2020-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01091-20"}}