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Here we show that West Nile virus (WNV) NS1\u2032 and NS1 localize to the same cellular compartments when expressed from plasmid DNAs and also colocalize to viral RNA replication sites in infected cells. Using complementation analysis with NS1-deleted WNV cDNA, we demonstrated that NS1\u2032 is able to substitute for the crucial function of NS1 in virus replication.<\/jats:p>","DOI":"10.1128\/jvi.01101-13","type":"journal-article","created":{"date-parts":[[2013,6,12]],"date-time":"2013-06-12T23:00:06Z","timestamp":1371078006000},"page":"9384-9390","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["NS1\u2032 Colocalizes with NS1 and Can Substitute for NS1 in West Nile Virus Replication"],"prefix":"10.1128","volume":"87","author":[{"given":"Lucy B.","family":"Young","sequence":"first","affiliation":[{"name":"Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezequiel","family":"Balmori Melian","sequence":"additional","affiliation":[{"name":"Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander A.","family":"Khromykh","sequence":"additional","affiliation":[{"name":"Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)59002-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01374-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2009.10.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1449"},{"key":"e_1_3_2_6_2","first-page":"253","article-title":"The ecology and epidemiology of Kunjin virus","volume":"267","author":"Hall RA","year":"2002","unstructured":"HallRA BroomAK SmithDW MackenzieJS. 2002. 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