{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:55:19Z","timestamp":1789649719051,"version":"4.0.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) uses dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN) to facilitate cell entry via cellular receptor-angiotensin-converting enzyme 2. For this project, we used recombinant baculoviruses expressing different lengths of SARS-CoV spike (S) protein in a capture assay to deduce the minimal DC-SIGN binding region. Our results identified the region location between amino acid (aa) residues 324 to 386 of the S protein. We then generated nine monoclonal antibodies (MAbs) against the S protein to map the DC-SIGN-binding domain using capture assays with pseudotyped viruses and observed that MAb SIa5 significantly blocked S protein-DC-SIGN interaction. An enhancement assay using the HKU39849 SARS-CoV strain and human immature dendritic cells confirmed our observation. Data from a pepscan analysis and M13 phage peptide display library system mapped the reactive MAb SIa5 epitope to aa residues 363 to 368 of the S protein. Results from a capture assay testing three pseudotyped viruses with mutated N-linked glycosylation sites of the S protein indicate that only two pseudotyped viruses (N330Q and N357Q, both of which lost glycosylation sites near the SIa5 epitope) had diminished DC-SIGN-binding capacity. We also noted that MAb SIb4 exerted a neutralizing effect against HKU39849; its reactive epitope was mapped to aa residues 435 to 439 of the S protein. We offer the data to facilitate the development of therapeutic agents and preventive vaccines against SARS-CoV infection.<\/jats:p>","DOI":"10.1128\/jvi.01138-06","type":"journal-article","created":{"date-parts":[[2006,10,15]],"date-time":"2006-10-15T06:42:36Z","timestamp":1160894556000},"page":"10315-10324","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Identifying Epitopes Responsible for Neutralizing Antibody and DC-SIGN Binding on the Spike Glycoprotein of the Severe Acute Respiratory Syndrome Coronavirus"],"prefix":"10.1128","volume":"80","author":[{"given":"Yi-Ping","family":"Shih","sequence":"first","affiliation":[{"name":"AIDS Prevention and Research Center, National Yang-Ming University"},{"name":"Division of Preventive Medicine, Institute of Public Health, School of Medicine National Yang-Ming University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Yen","family":"Chen","sequence":"additional","affiliation":[{"name":"AIDS Prevention and Research Center, National Yang-Ming University"},{"name":"Division of Preventive Medicine, Institute of Public Health, School of Medicine National Yang-Ming University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Jen","family":"Liu","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development Center, National Health Research Institutes"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan-Hsuan","family":"Chen","sequence":"additional","affiliation":[{"name":"AIDS Prevention and Research Center, National Yang-Ming University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Ming","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Preventive Medicine, Institute of Public Health, School of Medicine National Yang-Ming University"},{"name":"Department of Pathology and Laboratory Diagnosis, Taipei Veterans General Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chan","family":"Chao","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ming Arthur","family":"Chen","sequence":"additional","affiliation":[{"name":"AIDS Prevention and Research Center, National Yang-Ming University"},{"name":"Division of Preventive Medicine, Institute of Public Health, School of Medicine National Yang-Ming University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/282615a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.13.6841-6844.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.6.671"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1698"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.12.7602"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.4049\/jimmunol.147.7.2368","volume":"147","year":"1991","unstructured":"Chen, Y. 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