{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T20:39:14Z","timestamp":1774730354872,"version":"3.50.1"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The suppressor of cytokine signaling 1 (SOCS-1) protein modulates cytokine signaling by binding to and inhibiting the function of Janus kinases (JAKs), ErbB, and other tyrosine kinases. We have developed a small tyrosine kinase inhibitor peptide (Tkip) that binds to the autophosphorylation site of tyrosine kinases and inhibits activation of STAT transcription factors. We have also shown that a peptide corresponding to the kinase-inhibitory region of SOCS-1, SOCS1-KIR, similarly interacts with the activation loop of JAK2 and blocks STAT activation. Poxviruses activate cellular tyrosine kinases, such as ErbB-1 and JAK2, in the infection of cells. We used the pathogenesis of vaccinia virus in C57BL\/6 mice to determine the ability of the SOCS-1 mimetics to protect mice against lethal vaccinia virus infection. Injection of mice intraperitoneally with Tkip or SOCS1-KIR containing a palmitate for cell penetration, before and at the time of intranasal challenge with 2 \u00d7 10\n            <jats:sup>6<\/jats:sup>\n            PFU of vaccinia virus, resulted in complete protection at 100 \u03bcg. Initiation of treatment 1 day postinfection resulted in 80% survival. Administration of SOCS-1 mimetics by the oral route also protected mice against lethal effects of the virus. Both SOCS1-KIR and Tkip inhibited vaccinia virus transcription and replication at early and possibly later stages of infection. Vaccinia virus-induced phosphorylation of ErbB-1 and JAK2 was inhibited by the mimetics. Protected mice mounted a strong humoral and cellular response to vaccinia virus. The use of SOCS-1 mimetics in the treatment of poxvirus infections reveals an endogenous regulatory system that previously was not known to have an antiviral function.\n          <\/jats:p>","DOI":"10.1128\/jvi.01138-08","type":"journal-article","created":{"date-parts":[[2008,11,20]],"date-time":"2008-11-20T01:25:48Z","timestamp":1227144348000},"page":"1402-1415","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["SOCS-1 Mimetics Protect Mice against Lethal Poxvirus Infection: Identification of a Novel Endogenous Antiviral System"],"prefix":"10.1128","volume":"83","author":[{"given":"Chulbul M.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rea","family":"Dabelic","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian W.","family":"Waiboci","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsey D.","family":"Jager","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda L.","family":"Heron","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard M.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, P.O. 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