{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:42:50Z","timestamp":1761896570191,"version":"3.41.2"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2017,10,15]],"date-time":"2017-10-15T00:00:00Z","timestamp":1508025600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NS082228","AG032958"],"award-info":[{"award-number":["NS082228","AG032958"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NS093716"],"award-info":[{"award-number":["NS093716"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R25HL103286"],"award-info":[{"award-number":["R25HL103286"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2017,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The neurotropic herpesvirus varicella-zoster virus (VZV) establishes a lifelong latent infection in humans following primary infection. The low abundance of VZV nucleic acids in human neurons has hindered an understanding of the mechanisms that regulate viral gene transcription during latency. To overcome this critical barrier, we optimized a targeted capture protocol to enrich VZV DNA and cDNA prior to whole-genome\/transcriptome sequence analysis. Since the VZV genome is remarkably stable, it was surprising to detect that VZV32, a VZV laboratory strain with no discernible growth defect in tissue culture, contained a 2,158-bp deletion in open reading frame (ORF) 12. Consequently, ORF 12 and 13 protein expression was abolished and Akt phosphorylation was inhibited. The discovery of the ORF 12 deletion, revealed through targeted genome sequencing analysis, points to the need to authenticate the VZV genome when the virus is propagated in tissue culture.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>Viruses isolated from clinical samples often undergo genetic modifications when cultured in the laboratory. Historically, VZV is among the most genetically stable herpesviruses, a notion supported by more than 60 complete genome sequences from multiple isolates and following multiple<jats:italic>in vitro<\/jats:italic>passages. However, application of enrichment protocols to targeted genome sequencing revealed the unexpected deletion of a significant portion of VZV ORF 12 following propagation in cultured human fibroblast cells. While the enrichment protocol did not introduce bias in either the virus genome or transcriptome, the findings indicate the need for authentication of VZV by sequencing when the virus is propagated in tissue culture.<\/jats:p>","DOI":"10.1128\/jvi.01141-17","type":"journal-article","created":{"date-parts":[[2017,7,27]],"date-time":"2017-07-27T00:30:35Z","timestamp":1501115435000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Targeted Genome Sequencing Reveals Varicella-Zoster Virus Open Reading Frame 12 Deletion"],"prefix":"10.1128","volume":"91","author":[{"given":"Randall J.","family":"Cohrs","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"},{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Addilynn","family":"Beach","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bridget","family":"Sanford","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Hematology, Oncology, and Bone Marrow Transplant, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas L.","family":"Baird","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Como","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiharu","family":"Graybill","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dallas","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eden","family":"Tekeste","sequence":"additional","affiliation":[{"name":"Metropolitan State University, Denver, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell","family":"Ballard","sequence":"additional","affiliation":[{"name":"Metropolitan State University, Denver, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Yalacki","sequence":"additional","affiliation":[{"name":"Rice University, Houston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4058-3661","authenticated-orcid":false,"given":"Seth","family":"Frietze","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory and Radiation Sciences, University of Vermont, Burlington, Vermont, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tihana","family":"Lenac Rovis","sequence":"additional","affiliation":[{"name":"Center for Proteomics, Faculty of Medicine, Department of Histology and Embryology, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stipan","family":"Jonji\u0107","sequence":"additional","affiliation":[{"name":"Center for Proteomics, Faculty of Medicine, Department of Histology and Embryology, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Haas","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institut, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Munich, Germany"},{"name":"Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don","family":"Gilden","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"},{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.06.036"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.10.90"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01070-14"},{"key":"e_1_3_3_5_2","first-page":"17","article-title":"Latency of varicella zoster virus in dorsal root, cranial, and enteric ganglia in vaccinated children","volume":"123","author":"Gershon AA","year":"2012","unstructured":"Gershon AA Chen J Davis L Krinsky C Cowles R Reichard R Gershon M . 2012. Latency of varicella zoster virus in dorsal root, cranial, and enteric ganglia in vaccinated children. Trans Am Clin Climatol Assoc123:17\u201333.","journal-title":"Trans Am Clin Climatol Assoc"},{"key":"e_1_3_3_6_2","first-page":"341","article-title":"VZV T cell-mediated immunity","volume":"342","author":"Weinberg A","year":"2010","unstructured":"Weinberg A Levin MJ . 2010. VZV T cell-mediated immunity. Curr Top Microbiol Immunol342:341\u2013357.","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00137-6"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001919"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2015.09.015"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000216"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2016.07.035"},{"key":"e_1_3_3_12_2","first-page":"1692","article-title":"Varicella-zoster virus infections","volume":"21","author":"Gilden D","year":"2015","unstructured":"Gilden D . 2015. Varicella-zoster virus infections. Continuum (Minneap Minn)21:1692\u20131703.","journal-title":"Continuum (Minneap Minn)"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.24.11464-11471.2000"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00445-11"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06810-11"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053010"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/v5092106"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2014.04.014"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00594-15"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003512"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13365-012-0142-x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004885"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522575113"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027805"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.5.2789-2796.1996"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01331-12"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13365-012-0123-0"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01862-10"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02745-06"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.11.019"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.017814-0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00437-15"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01145-07"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.05.004"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-370"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02337-14"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw398"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00357-12"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/838241"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23344"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu406"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000208"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.13.6799-6807.2004"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.09.037"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-008-0197-4"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.21.11718-11732.2003"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02395-12"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01546-16"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06923-11"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0507"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-68-5-1449"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.15.7376"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.10.039"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00878-10"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80946-0"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00476-14"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003896"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00500-14"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(88)90034-2"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.19.1.199-203.1978"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050364"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01317286"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-60-2-371"},{"key":"e_1_3_3_64_2","doi-asserted-by":"crossref","first-page":"416","DOI":"10.4049\/jimmunol.91.3.416","article-title":"Biological characteristics of a continuous kidney cell line derived from the African green monkey","volume":"91","author":"Hopps HE","year":"1963","unstructured":"Hopps HE Bernheim BC Nisalak A Tjio JH Smadel JE . 1963. Biological characteristics of a continuous kidney cell line derived from the African green monkey. J Immunol91:416\u2013424.","journal-title":"J Immunol"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21173"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02617-15"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(82)90073-8"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01141-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01141-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T20:25:17Z","timestamp":1719347117000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01141-17"}},"subtitle":[],"editor":[{"given":"Rozanne M.","family":"Sandri-Goldin","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,10,15]]},"references-count":66,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2017,10,15]]}},"alternative-id":["10.1128\/JVI.01141-17"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01141-17","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"type":"print","value":"0022-538X"},{"type":"electronic","value":"1098-5514"}],"subject":[],"published":{"date-parts":[[2017,10,15]]},"assertion":[{"value":"2017-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01141-17"}}