{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:55:05Z","timestamp":1791114905219,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,1,15]],"date-time":"2010-01-15T00:00:00Z","timestamp":1263513600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Rift Valley fever virus (RVFV) is an emerging, highly pathogenic virus; RVFV infection can lead to encephalitis, retinitis, or fatal hepatitis associated with hemorrhagic fever in humans, as well as death, abortions, and fetal deformities in animals. RVFV nonstructural NSs protein, a major factor of the virulence, forms filamentous structures in the nuclei of infected cells. In order to further understand RVFV pathology, we investigated, by chromatin immunoprecipitation, immunofluorescence, fluorescence in situ hybridization, and confocal microscopy, the capacity of NSs to interact with the host genome. Our results demonstrate that even though cellular DNA is predominantly excluded from NSs filaments, NSs interacts with some specific DNA regions of the host genome such as clusters of pericentromeric \u03b3-satellite sequence. Targeting of these sequences by NSs was correlated with the induction of chromosome cohesion and segregation defects in RVFV-infected murine, as well as sheep cells. Using recombinant nonpathogenic virus rZH\u0394NSs210-230, expressing a NSs protein deleted of its region of interaction with cellular factor SAP30, we showed that the NSs-SAP30 interaction was essential for NSs to target pericentromeric sequences, as well as for induction of chromosome segregation defects. The effect of RVFV upon the inheritance of genetic information is discussed with respect to the pathology associated with fetal deformities and abortions, highlighting the main role played by cellular cofactor SAP30 on the establishment of NSs interactions with host DNA sequences and RVFV pathogenesis.<\/jats:p>","DOI":"10.1128\/jvi.01165-09","type":"journal-article","created":{"date-parts":[[2009,11,5]],"date-time":"2009-11-05T02:16:36Z","timestamp":1257387396000},"page":"928-939","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Nonstructural NSs Protein of Rift Valley Fever Virus Interacts with Pericentromeric DNA Sequences of the Host Cell, Inducing Chromosome Cohesion and Segregation Defects"],"prefix":"10.1128","volume":"84","author":[{"given":"Z.","family":"Mansuroglu","sequence":"first","affiliation":[{"name":"Re\u0301gulation de la Transcription et Maladies Ge\u0301ne\u0301tiques, CNRS UPR2228, Universite\u0301 Paris Descartes, 45 Rue des Saints Pe\u0300res, 75270 Paris Cedex 06, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Josse","sequence":"additional","affiliation":[{"name":"Re\u0301gulation de la Transcription et Maladies Ge\u0301ne\u0301tiques, CNRS UPR2228, Universite\u0301 Paris Descartes, 45 Rue des Saints Pe\u0300res, 75270 Paris Cedex 06, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Gilleron","sequence":"additional","affiliation":[{"name":"Physiopathologie du Contro\u0302le de la Prolife\u0301ration Germinale, INSERM S895, Universite\u0301 Paris Descartes, 45 Rue des Saints Pe\u0300res, 75270 Paris Cedex 06, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Billecocq","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire des Bunyavirus, Institut Pasteur, 25 Rue du Docteur Roux, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Leger","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire des Bunyavirus, Institut Pasteur, 25 Rue du Docteur Roux, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bouloy","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire des Bunyavirus, Institut Pasteur, 25 Rue du Docteur Roux, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Bonnefoy","sequence":"additional","affiliation":[{"name":"Re\u0301gulation de la Transcription et Maladies Ge\u0301ne\u0301tiques, CNRS UPR2228, Universite\u0301 Paris Descartes, 45 Rue des Saints Pe\u0300res, 75270 Paris Cedex 06, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21132"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.9798-9806.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.05.033"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.01.046"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02501-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_3_2_8_2","first-page":"614","year":"2000","unstructured":"Elliott, R. 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