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The present report demonstrates, in a model system, profound mutual effects of mycoplasma and cardioviruses in animal cell cultures. In contrast to mycoplasma-free cells, cultures contaminated with\n            <jats:italic>Mycoplasma hyorhinis<\/jats:italic>\n            responded to infection with encephalomyocarditis virus (EMCV), a picornavirus, but not with poliovirus (also a picornavirus), with a strong activation of a DNase(s), as evidenced by the TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) immunofluorescence assay and electrophoretic analysis of host DNA. This degradation was reminiscent of that observed upon apoptosis but was caspase independent, judging by the failure of the specific pan-caspase inhibitor Q-VD-OPh to prevent it. The electrophoretic mobility of the enzyme responsible for DNA degradation and dependence of its activity on ionic conditions strongly suggested that it was represented by a DNase(s) of mycoplasma origin. In cells not infected with EMCV, the relevant DNase was dormant. The possibility is discussed that activation of the mycoplasma DNase might be linked to a relatively early increase in permeability of plasma membrane of the infected cells caused by EMCV. This type of unanticipated virus-mycoplasma \u201ccooperation\u201d may exemplify the complexity of pathogen-host interactions under conditions when viruses and microorganisms are infecting the same host. In the course of the present study, it was also demonstrated that pan-caspase inhibitor zVAD(OMe).fmk strongly suppressed cardiovirus polyprotein processing, illustrating an additional pitfall in investigations of viral effects on the apoptotic system of host cells.\n          <\/jats:p>","DOI":"10.1128\/jvi.01167-09","type":"journal-article","created":{"date-parts":[[2009,7,16]],"date-time":"2009-07-16T00:59:43Z","timestamp":1247705983000},"page":"9940-9951","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Interactions between Viral and Prokaryotic Pathogens in a Mixed Infection with Cardiovirus and Mycoplasma"],"prefix":"10.1128","volume":"83","author":[{"given":"Peter V.","family":"Lidsky","sequence":"first","affiliation":[{"name":"M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyudmila I.","family":"Romanova","sequence":"additional","affiliation":[{"name":"M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina S.","family":"Kolesnikova","sequence":"additional","affiliation":[{"name":"M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryana V.","family":"Bardina","sequence":"additional","affiliation":[{"name":"M. P. 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