{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:09:05Z","timestamp":1789988945925,"version":"4.0.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI141889"],"award-info":[{"award-number":["R01AI141889"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008213","name":"South Dakota State University","doi-asserted-by":"publisher","award":["AES 3AH-673"],"award-info":[{"award-number":["AES 3AH-673"]}],"id":[{"id":"10.13039\/100008213","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>Influenza D virus (IDV) is a new type of influenza virus that uses cattle as the primary reservoir and infects multiple agricultural animals. Increased outbreaks in pigs and serological and genetic evidence of human infection have raised concerns about potential IDV adaptation in humans. Here, we have developed a plasmid-based IDV reverse-genetics system that can generate infectious viruses with replication kinetics similar to those of wild-type viruses following transfection of cultured cells. Further characterization demonstrated that viruses rescued from the described RGS resembled the parental viruses in biological and receptor-binding properties. We also developed and validated an IDV minireplicon reporter system that specifically measures viral RNA polymerase activity. In summary, the reverse-genetics system and minireplicon reporter assay described in this study should be of value in identifying viral determinants of cross-species transmission and pathogenicity of novel influenza D viruses.<\/jats:p>","DOI":"10.1128\/jvi.01186-19","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T10:23:25Z","timestamp":1565778205000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Development and Characterization of a Reverse-Genetics System for Influenza D Virus"],"prefix":"10.1128","volume":"93","author":[{"given":"Jieshi","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runxia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"},{"name":"Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsui-wen","family":"Chou","sequence":"additional","affiliation":[{"name":"Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Ghedin","sequence":"additional","affiliation":[{"name":"Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zizhang","family":"Sheng","sequence":"additional","affiliation":[{"name":"Zuckerman Mind Brian Behavior Institute, Columbia University, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-lun","family":"Zhai","sequence":"additional","affiliation":[{"name":"Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Disease Prevention, Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7263-8420","authenticated-orcid":false,"given":"Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA"},{"name":"BioSNTR, Brookings, South Dakota, USA"},{"name":"SD-CBRC, Brookings, South Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA"},{"name":"BioSNTR, Brookings, South Dakota, USA"},{"name":"SD-CBRC, Brookings, South Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-018-3708-y"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.15.120"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-015-0193-x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003176"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2202.151439"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-014-1107-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-12012-3"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2407.171937"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2308.170059"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2102.141449"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2402.170759"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2208.160362"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.08.030"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000492"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.11.004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.09.005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00031-14"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2017.1365216"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2309.170342"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.05.017"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12423"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201295"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-018-0071-7"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2018.00174"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03122-15"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01853-18"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/med.21401"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005411"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.666"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199227"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100133697"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.4172\/1747-0862.1000042"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505587102"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2004.02.028"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.07.021"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2012.00392.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172393399"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00910-07"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9679-9682.1999"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82452-0"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.6.4188-4192.1996"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/128_2013_466"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature15525"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1244730"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01925-12"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00254-17"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-29"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01445-08"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8080218"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.04.001"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01186-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01186-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T14:52:26Z","timestamp":1646491946000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01186-19"}},"subtitle":[],"editor":[{"given":"Rebecca Ellis","family":"Dutch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":51,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1128\/JVI.01186-19"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01186-19","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"2019-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01186-19"}}