{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:39:57Z","timestamp":1791189597349,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rift Valley fever virus (RVFV) is a member of the\n            <jats:italic>Bunyaviridae<\/jats:italic>\n            virus family (genus\n            <jats:italic>Phlebovirus<\/jats:italic>\n            ) and is considered to be one of the most important pathogens in Africa, causing viral zoonoses in livestock and humans. Here, we report the characterization of the three-dimensional structural organization of RVFV vaccine strain MP-12 by cryoelectron tomography. Vitrified-hydrated virions were found to be spherical, with an average diameter of 100 nm. The virus glycoproteins formed cylindrical hollow spikes that clustered into distinct capsomeres. In contrast to previous assertions that RVFV is pleomorphic, the structure of RVFV MP-12 was found to be highly ordered. The three-dimensional map was resolved to a resolution of 6.1 nm, and capsomeres were observed to be arranged on the virus surface in an icosahedral lattice with clear T=12 quasisymmetry. All icosahedral symmetry axes were visible in self-rotation functions calculated using the Fourier transform of the RVFV MP-12 tomogram. To the best of our knowledge, a triangulation number of 12 had previously been reported only for Uukuniemi virus, a bunyavirus also within the\n            <jats:italic>Phlebovirus<\/jats:italic>\n            genus. The results presented in this study demonstrate that RVFV MP-12 possesses T=12 icosahedral symmetry and suggest that other members of the\n            <jats:italic>Phlebovirus<\/jats:italic>\n            genus, as well as of the\n            <jats:italic>Bunyaviridae<\/jats:italic>\n            family, may adopt icosahedral symmetry. Knowledge of the virus architecture may provide a structural template to develop vaccines and diagnostics, since no effective anti-RVFV treatments are available for human use.\n          <\/jats:p>","DOI":"10.1128\/jvi.01191-08","type":"journal-article","created":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T00:15:13Z","timestamp":1219277713000},"page":"10341-10348","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":133,"title":["Three-Dimensional Organization of Rift Valley Fever Virus Revealed by Cryoelectron Tomography"],"prefix":"10.1128","volume":"82","author":[{"given":"Alexander N.","family":"Freiberg","sequence":"first","affiliation":[{"name":"Department of Pathology, Center for Biodefense and Emerging Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B.","family":"Sherman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology"},{"name":"Sealy Center for Structural Biology and Molecular Biophysics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc C.","family":"Morais","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology"},{"name":"Sealy Center for Structural Biology and Molecular Biophysics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Holbrook","sequence":"additional","affiliation":[{"name":"Department of Pathology, Center for Biodefense and Emerging Infectious Diseases"},{"name":"Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas 77555"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley J.","family":"Watowich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology"},{"name":"Sealy Center for Structural Biology and Molecular Biophysics"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(02)00378-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.12.046"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-66-10-2271"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1962.027.001.005"},{"key":"e_1_3_2_6_2","first-page":"982","volume":"49","year":"2000","unstructured":"Centers for Disease Control and Prevention. 2000. 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