{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:22:59Z","timestamp":1791397379835,"version":"4.3.3"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The\nEpstein-Barr virus (EBV) latent cycle promoter Wp, present in each\ntandemly arrayed copy of the BamHI W region in the EBV genome, drives\nexpression of the EB viral nuclear antigens (EBNAs) at the initiation\nof virus-induced B-cell transformation. Thereafter, an alternative EBNA\npromoter, Cp, becomes dominant, Wp activity declines dramatically, and\nbisulfite sequencing of EBV-transformed lymphoblastoid cell lines\n(LCLs) shows extensive Wp methylation. Despite this, Wp is never\ncompletely silenced in LCLs. Here, using a combination of bisulfite\nsequencing and methylation-specific PCR, we show that in standard LCLs\ntransformed with wild-type EBV isolates, some Wp copies always remain\nunmethylated, and in LCLs transformed with a recombinant EBV carrying\njust two BamHI W copies, Wp is completely unmethylated. Furthermore, we\nhave analyzed rare LCLs, recently established using wild-type EBV\nisolates, and rare Burkitt lymphoma (BL) cell clones, recently\nestablished from tumors carrying EBNA2-deleted EBV genomes, which\nexpress EBNAs exclusively from Wp-initiated transcripts. Here, in sharp\ncontrast to standard LCL and BL lines, all resident copies of Wp appear\nto be predominantly hypomethylated. Thus, studies of B cells with\natypical patterns of Wp usage emphasize the strong correlation between\nthe presence of unmethylated Wp sequences and promoter\nactivity.<\/jats:p>","DOI":"10.1128\/jvi.01204-06","type":"journal-article","created":{"date-parts":[[2006,10,15]],"date-time":"2006-10-15T06:42:36Z","timestamp":1160894556000},"page":"10700-10711","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Methylation Status of theEpstein-Barr Virus (EBV) BamHI W Latent Cycle Promoter and Promoter Activity: Analysis with Novel EBV-Positive Burkitt and LymphoblastoidCell Lines"],"prefix":"10.1128","volume":"80","author":[{"given":"Isabel A.","family":"Hutchings","sequence":"first","affiliation":[{"name":"Cancer\rResearch UK Institute for Cancer Studies, The University of Birmingham,\rEdgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary J.","family":"Tierney","sequence":"additional","affiliation":[{"name":"Cancer\rResearch UK Institute for Cancer Studies, The University of Birmingham,\rEdgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma L.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Cancer\rResearch UK Institute for Cancer Studies, The University of Birmingham,\rEdgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julianna","family":"Stylianou","sequence":"additional","affiliation":[{"name":"Cancer\rResearch UK Institute for Cancer Studies, The University of Birmingham,\rEdgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan B.","family":"Rickinson","sequence":"additional","affiliation":[{"name":"Cancer\rResearch UK Institute for Cancer Studies, The University of Birmingham,\rEdgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew I.","family":"Bell","sequence":"additional","affiliation":[{"name":"Cancer\rResearch UK Institute for Cancer Studies, The University of Birmingham,\rEdgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.5.2126-2134.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.14.8166-8172.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90893-G"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90561-8"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910450623"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030404"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/310207a0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9440"},{"key":"e_1_3_2_10_2","unstructured":"Bell A. 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