{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:03:06Z","timestamp":1790517786136,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T00:00:00Z","timestamp":1546560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T00:00:00Z","timestamp":1547510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["200187\/Z\/15\/Z"],"award-info":[{"award-number":["200187\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"name":"HPRU in Respiratory Infections"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,1,15]]},"abstract":"<jats:p>New antiviral drugs are needed as a first line of defense in the event of a novel influenza pandemic. Favipiravir is a broad-spectrum antiviral which is effective against influenza. The exact mechanism of how favipiravir works to inhibit influenza is still unclear. We used next-generation sequencing (NGS) to demonstrate that favipiravir causes mutations in influenza RNA. The greater depth of NGS sequence information over traditional sequencing methods allowed us to precisely determine the bias of particular mutations caused by favipiravir. NGS can also be used in a standard diagnostic pipeline to show that favipiravir is acting on the virus by revealing the mutation bias pattern typical to the drug. Our work will aid in testing whether viruses are resistant to favipiravir and may help demonstrate the effect of favipiravir on viruses in a clinical setting. This will be important if favipiravir is used during a future influenza pandemic.<\/jats:p>","DOI":"10.1128\/jvi.01217-18","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T09:10:14Z","timestamp":1540804214000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Determining the Mutation Bias of Favipiravir in Influenza Virus Using Next-Generation Sequencing"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4597-5963","authenticated-orcid":false,"given":"Daniel H.","family":"Goldhill","sequence":"first","affiliation":[{"name":"Public Health England, London, United Kingdom"},{"name":"Department of Virology, Faculty of Medicine, Imperial College, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinky","family":"Langat","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Imperial College, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyao","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Imperial College, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Galiano","sequence":"additional","affiliation":[{"name":"Public Health England, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahjahan","family":"Miah","sequence":"additional","affiliation":[{"name":"Public Health England, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Kellam","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Imperial College, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Zambon","sequence":"additional","affiliation":[{"name":"Public Health England, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angie","family":"Lackenby","sequence":"additional","affiliation":[{"name":"Public Health England, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy S.","family":"Barclay","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Imperial College, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"World Health Organization. 2018. Influenza (seasonal) factsheet. World Health Organization Geneva Switzerland. www.who.int\/en\/news-room\/fact-sheets\/detail\/influenza-(seasonal)."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04795"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/507550"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/518936"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.04.009"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp0900648"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12392"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1239844"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.09.008"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12446"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00102-15"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.977-981.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01051-06"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909603107"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.02.198"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01739-09"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.09.015"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.93.027"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01219-10"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001342"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2014.02.014"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep14775"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv522"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00061-15"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.03.003"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.981-986.2005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02346-12"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.03679"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164691"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185998"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01156-16"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00649-13"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068347"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.04.005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep26742"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku209"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002535"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110064108"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00522-15"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006796"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep04942"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8060155"},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","unstructured":"Goldhill DH Langat P Xie H Galiano M Shahjahan M Kellam P Lackenby A Barclay W. 2018. Determining the mutation bias of favipiravir in influenza virus using next-generation sequencing. bioRxiv 10.1101\/375378.","DOI":"10.1101\/375378"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","unstructured":"Pauly MD Procario M Lauring AS. 2017. The mutation rates and mutational bias of influenza A virus. bioRxiv doi:10.1101\/110197.","DOI":"10.1101\/110197"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005856"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03483-14"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0205"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203613109"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16474"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01109-09"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01217-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01217-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:52:01Z","timestamp":1646473921000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01217-18"}},"subtitle":[],"editor":[{"given":"Adolfo","family":"Garc\u00eda-Sastre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,1,15]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,1,15]]}},"alternative-id":["10.1128\/JVI.01217-18"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01217-18","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/375378","asserted-by":"object"}]},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,15]]},"assertion":[{"value":"2018-07-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01217-18"}}