{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T11:39:28Z","timestamp":1791027568184,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A monoclonal Fab (Fab 3674) selected from a human nonimmune phage library by panning against the chimeric construct N\n            <jats:sub>CCG<\/jats:sub>\n            -gp41 (which comprises an exposed coiled-coil trimer of gp41 N helices fused in the helical phase onto the minimal thermostable ectodomain of gp41) is described. Fab 3674 is shown to neutralize diverse laboratory-adapted B strains of human immunodeficiency virus type 1 (HIV-1) and primary isolates of subtypes A, B, and C in an Env-pseudotyped-virus neutralization assay, albeit with reduced potency (approximately 25-fold) compared to that of 2F5 and 4E10. Alanine scanning mutagenesis maps a novel epitope to a shallow groove on the N helices of gp41 that is exposed between two C helices in the fusogenic six-helix bundle conformation of gp41. Bivalent Fab 3674 and the C34 peptide (a potent fusion inhibitor derived from the C helix of gp41) are shown to act at similar stages of the fusion reaction and to neutralize HIV-1 synergistically, providing additional evidence that the epitope of Fab 3674 is new and distinct from the binding site of C34.\n          <\/jats:p>","DOI":"10.1128\/jvi.01260-07","type":"journal-article","created":{"date-parts":[[2007,9,27]],"date-time":"2007-09-27T00:44:20Z","timestamp":1190853860000},"page":"12946-12953","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["A Monoclonal Fab Derived from a Human Nonimmune Phage Library Reveals a New Epitope on gp41 and Neutralizes Diverse Human Immunodeficiency Virus Type 1 Strains"],"prefix":"10.1128","volume":"81","author":[{"given":"Elena","family":"Gustchina","sequence":"first","affiliation":[{"name":"Laboratories of Chemical Physics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Louis","sequence":"additional","affiliation":[{"name":"Laboratories of Chemical Physics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Son N.","family":"Lam","sequence":"additional","affiliation":[{"name":"Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole A.","family":"Bewley","sequence":"additional","affiliation":[{"name":"Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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