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Viral entry is mediated by the arenavirus GP complex, which consists of the stable signal peptide (SSP), the receptor-binding subunit GP1, and the transmembrane subunit GP2. The cytoplasmic tail (CT) of GP2 is highly conserved among arenaviruses, but its functional role in viral replication is not completely understood. Using a reverse genetics system of a prototypic arenavirus, Pichinde virus (PICV), we show that the GP2 CT contains certain conserved residues that are essential for virus replication, implicating it as a potentially good target for developing antivirals and live-attenuated viral vaccines against deadly arenavirus pathogens.<\/jats:p>","DOI":"10.1128\/jvi.01277-19","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T09:46:45Z","timestamp":1566812805000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Biological Characterization of Conserved Residues within the Cytoplasmic Tail of the Pichinde Arenaviral Glycoprotein Subunit 2 (GP2)"],"prefix":"10.1128","volume":"93","author":[{"given":"Junjie","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Di","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mythili","family":"Dileepan","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan","family":"Brisse","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8271-2033","authenticated-orcid":false,"given":"Hinh","family":"Ly","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2012.12.003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/v5010241"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.456"},{"key":"e_1_3_2_5_2","unstructured":"WHO. 14 February 2019. 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