{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T19:24:19Z","timestamp":1766690659857,"version":"3.41.2"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T00:00:00Z","timestamp":1601337600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Key R&D Plan of China","award":["2017YFD0501101"],"award-info":[{"award-number":["2017YFD0501101"]}]},{"name":"Major S&T Project of Hubei Province","award":["2017ABA138"],"award-info":[{"award-number":["2017ABA138"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872485","31941005","31672566"],"award-info":[{"award-number":["31872485","31941005","31672566"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,9,29]]},"abstract":"<jats:p>\n            Currently, the active-site residues and substrate specificities of 3C-like protease (3CL\n            <jats:sup>pro<\/jats:sup>\n            ) differ among nidoviruses, and the detailed catalytic mechanism remains largely unknown. Here, porcine torovirus (PToV) 3CL\n            <jats:sup>pro<\/jats:sup>\n            cleaves 12 sites in the polyproteins, including its N- and C-terminal self-processing sites. Unlike coronaviruses and arteriviruses, PToV 3CL\n            <jats:sup>pro<\/jats:sup>\n            employed His53 and Ser160 as the active-site residues that recognize a glutamine (Gln) at the P1 position. Surprisingly, mutations of P1-Gln impaired the C-terminal self-processing but did not affect N-terminal self-processing. The \u201cnoncanonical\u201d substrate specificity for its N-terminal self-processing was attributed to the phenylalanine (Phe) residue at the P4 position in the N-terminal site. Furthermore, a double glycine (neutral) substitution at the putative P4-Phe-binding residues (P62G\/L185G) abolished the cleavage activity of PToV 3CL\n            <jats:sup>pro<\/jats:sup>\n            suggested the potential hydrophobic force between the PToV 3CL\n            <jats:sup>pro<\/jats:sup>\n            and P4-Phe side chains.\n          <\/jats:p>","DOI":"10.1128\/jvi.01282-20","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T14:03:57Z","timestamp":1596031437000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of Self-Processing Activities and Substrate Specificities of Porcine Torovirus 3C-Like Protease"],"prefix":"10.1128","volume":"94","author":[{"given":"Shangen","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwei","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjin","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Tong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zixin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahui","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyao","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1406-6409","authenticated-orcid":false,"given":"Liurong","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3394-3702","authenticated-orcid":false,"given":"Dang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0023-9188","authenticated-orcid":false,"given":"Shaobo","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China"},{"name":"The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-64-9-1849"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(82)90036-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)91454-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01484-14"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(84)90014-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.10.2738-2744.1993"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.25.4.637-640.1987"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.5.3507-3511.1998"},{"key":"e_1_3_2_10_2","first-page":"876","article-title":"Viruses and virus-like particles detected during examination of feces from calves and piglets with diarrhea","volume":"30","author":"Durham PJK","year":"1989","unstructured":"Durham PJK, Hassard LE, Norman GR, Yemen RL. 1989. Viruses and virus-like particles detected during examination of feces from calves and piglets with diarrhea. Can Vet J 30:876\u2013881.","journal-title":"Can Vet J"},{"key":"e_1_3_2_11_2","first-page":"102","article-title":"Breda virus-like particles in pigs in South Africa","volume":"63","author":"Penrith ML","year":"1992","unstructured":"Penrith ML, Gerdes GH. 1992. Breda virus-like particles in pigs in South Africa. J South Afr Vet Assoc 63:102.","journal-title":"J South Afr Vet Assoc"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.02.019"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.10.031"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-010-0595-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-019-04514-4"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.12.013"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.17.9567-9577.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13073"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190819"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000799"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/ve\/vex024"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-013-1861-x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-4-853"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)80109-7"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01461"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02040-14"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01758-06"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.8.4157-4167.2006"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01716-10"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.07.062"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.9.4864"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206978200"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf327"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310875200"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi036022q"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.1.177-185.1999"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82269-0"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8503"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002215"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.7872"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)80095-3"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560040801"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.71.5.3992-3997.1997"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2005.07.005"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2017.11.031"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2014.12.027"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.05.063"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-3-581"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2006.01.017"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature20167"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1601327113"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb3405"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.2.1415-1426.2003"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-3-595"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.0c00110"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2019.05.001"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000458"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.003413"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx108"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msn067"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1626"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.softx.2015.06.001"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901624RR"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz268"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky426"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01282-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01282-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:01:31Z","timestamp":1646492491000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01282-20"}},"subtitle":[],"editor":[{"given":"Tom","family":"Gallagher","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,9,29]]},"references-count":66,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2020,9,29]]}},"alternative-id":["10.1128\/JVI.01282-20"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01282-20","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"type":"print","value":"0022-538X"},{"type":"electronic","value":"1098-5514"}],"subject":[],"published":{"date-parts":[[2020,9,29]]},"assertion":[{"value":"2020-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01282-20"}}