{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T13:53:58Z","timestamp":1791467638143,"version":"4.3.4"},"reference-count":22,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["75N93021C00015"],"award-info":[{"award-number":["75N93021C00015"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["5030-32000-231-000-D"],"award-info":[{"award-number":["5030-32000-231-000-D"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Highly pathogenic avian influenza (HPAI) clade 2.3.4.4b H5N1 circulated widely in lactating dairy cows in the United States following the initial detection in 2024. Due to the critical need for intervention strategies for this outbreak, we evaluated antibody and cellular immune responses of a clade 2.3.4.4b H5 mRNA-LNP vaccine in calves. We found that the H5 mRNA-LNP vaccine induced a robust antibody and CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cell-mediated immune response and conferred protection against clade 2.3.4.4b H5N1 infection.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>It is important to develop vaccines that limit clade 2.3.4.4b H5N1 infection in cattle. In this study, we found that a new H5 mRNA-lipid nanoparticle vaccine is immunogenic when delivered intramuscularly to male Holstein calves.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.01289-26","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T13:00:17Z","timestamp":1791464417000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["H5 influenza virus mRNA-lipid nanoparticle (LNP) vaccination elicits adaptive immune responses in Holstein calves"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3082-0398","authenticated-orcid":true,"given":"Carine K.","family":"Souza","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/001ke5c68","id-type":"ROR","asserted-by":"publisher"}],"name":"National Animal Disease Center\u2014United States Department of Agriculture, Agricultural Research Service","place":["Ames, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5895-4163","authenticated-orcid":true,"given":"Jefferson J. 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