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We found that introduction of avian-virus-like amino acids at five other HA positions (positions 62, 81, 92, 144, and 193) of A\/Hong Kong\/1\/1968 virus decreased viral replication in human cells and transmission in pigs. Thus, substitutions at some of these positions facilitated emergence of the pandemic virus.<\/jats:p>","DOI":"10.1128\/jvi.01292-15","type":"journal-article","created":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T03:38:17Z","timestamp":1442461097000},"page":"12211-12216","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Role of Substitutions in the Hemagglutinin in the Emergence of the 1968 Pandemic Influenza Virus"],"prefix":"10.1128","volume":"89","author":[{"given":"Sjouke","family":"Van Poucke","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Doedt","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Baumann","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Matrosovich","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Dieter","family":"Klenk","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristien","family":"Van Reeth","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail","family":"Matrosovich","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.56.1.152-179.1992"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.05.009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201000118"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.07.003"},{"key":"e_1_3_2_6_2","first-page":"95","volume-title":"Influenza virology: current topics","author":"Matrosovich MN","year":"2006","unstructured":"Matrosovich MN, Klenk H-D, Kawaoka Y. 2006. 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