{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:27:23Z","timestamp":1791433643077,"version":"4.3.3"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2019,12,12]],"date-time":"2019-12-12T00:00:00Z","timestamp":1576108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001381","name":"National Research Foundation Singapore","doi-asserted-by":"publisher","award":["NRF20016NRF-CRP001-063"],"award-info":[{"award-number":["NRF20016NRF-CRP001-063"]}],"id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,12,12]]},"abstract":"<jats:p>\n            Flaviviruses include important human pathogens, such as dengue virus and Zika virus. NS5 is a nonstructural protein essential for flavivirus RNA replication with dual MTase and RdRp enzyme activities and thus constitutes a major drug target. Insights into NS5 structure, dynamics, and evolution should inform the development of antiviral inhibitors and vaccine design. We found that NS5 from DENV2 can adopt a conformation resembling that of NS5 from JEV and ZIKV. Replacement of the DENV2 NS5 linker with the JEV and ZIKV NS5 linkers abolished DENV2 replication in cells, without significantly impacting\n            <jats:italic>in vitro<\/jats:italic>\n            DENV2 NS5 enzymatic activities. We propose that heterotypic flavivirus NS5 linkers impede DENV2 NS5 protein-protein interactions that are essential for virus replication.\n          <\/jats:p>","DOI":"10.1128\/jvi.01294-19","type":"journal-article","created":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T11:43:36Z","timestamp":1570448616000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["NS5 from Dengue Virus Serotype 2 Can Adopt a Conformation Analogous to That of Its Zika Virus and Japanese Encephalitis Virus Homologues"],"prefix":"10.1128","volume":"94","author":[{"given":"Abbas","family":"El Sahili","sequence":"first","affiliation":[{"name":"NTU Institute of Structural Biology, School of Biological Sciences, Nanyang Technological University, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingjin Sherryl","family":"Soh","sequence":"additional","affiliation":[{"name":"NTU Institute of Structural Biology, School of Biological Sciences, Nanyang Technological University, Singapore"},{"name":"Novartis Institute for Tropical Diseases, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Schiltz","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Singapore"},{"name":"Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of L\u00fcbeck, L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u00efcha","family":"Gharbi-Ayachi","sequence":"additional","affiliation":[{"name":"NTU Institute of Structural Biology, School of Biological Sciences, Nanyang Technological University, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheah Chen","family":"Seh","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yong","family":"Shi","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siew Pheng","family":"Lim","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Lescar","sequence":"additional","affiliation":[{"name":"NTU Institute of Structural Biology, School of Biological Sciences, Nanyang Technological University, Singapore"},{"name":"Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)00146-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30010-X"},{"key":"e_1_3_2_4_2","unstructured":"WHO. 2017. 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