{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:52:01Z","timestamp":1790664721680,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100011102","name":"European Union Seventh Framework Programme","doi-asserted-by":"crossref","award":["316655"],"award-info":[{"award-number":["316655"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006544","name":"Israeli Ministry of Health","doi-asserted-by":"crossref","award":["310998"],"award-info":[{"award-number":["310998"]}],"id":[{"id":"10.13039\/501100006544","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["275\/13"],"award-info":[{"award-number":["275\/13"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human cytomegalovirus (HCMV) is the leading cause of congenital infection and is associated with a wide range of neurodevelopmental disabilities and intrauterine growth restriction. Yet our current understanding of the mechanisms modulating transplacental HCMV transmission is poor. The placenta, given its critical function in protecting the fetus, has evolved effective yet largely uncharacterized innate immune barriers against invading pathogens. Here we show that the intrinsic cellular restriction factor\n            <jats:underline>apo<\/jats:underline>\n            lipoprotein B\n            <jats:underline>e<\/jats:underline>\n            diting\n            <jats:underline>c<\/jats:underline>\n            atalytic subunit-like 3A (APOBEC3A [A3A]) is profoundly upregulated following\n            <jats:italic>ex vivo<\/jats:italic>\n            HCMV infection in human decidual tissues\u2014constituting the maternal aspect of the placenta. We directly demonstrated that A3A severely restricted HCMV replication upon controlled overexpression in epithelial cells, acting by a cytidine deamination mechanism to introduce hypermutations into the viral genome. Importantly, we further found that A3 editing of HCMV DNA occurs both\n            <jats:italic>ex vivo<\/jats:italic>\n            in HCMV-infected decidual organ cultures and\n            <jats:italic>in vivo<\/jats:italic>\n            in amniotic fluid samples obtained during natural congenital infection. Our results reveal a previously unexplored role for A3A as an innate anti-HCMV effector, activated by HCMV infection in the maternal-fetal interface. These findings pave the way to new insights into the potential impact of APOBEC proteins on HCMV pathogenesis.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            In view of the grave outcomes associated with congenital HCMV infection, there is an urgent need to better understand the innate mechanisms acting to limit transplacental viral transmission. Toward this goal, our findings reveal the role of the intrinsic cellular restriction factor A3A (which has never before been studied in the context of HCMV infection and vertical viral transmission) as a potent anti-HCMV innate barrier, activated by HCMV infection in the authentic tissues of the maternal-fetal interface. The detection of naturally occurring hypermutations in clinical amniotic fluid samples of congenitally infected fetuses further supports the idea of the occurrence of A3 editing of the viral genome in the setting of congenital HCMV infection. Given the widely differential tissue distribution characteristics and biological functions of the members of the A3 protein family, our findings should pave the way to future studies examining the potential impact of A3A as well as of other A3s on HCMV pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/jvi.01296-17","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T00:40:20Z","timestamp":1506559220000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["APOBEC3A Is Upregulated by Human Cytomegalovirus (HCMV) in the Maternal-Fetal Interface, Acting as an Innate Anti-HCMV Effector"],"prefix":"10.1128","volume":"91","author":[{"given":"Yiska","family":"Weisblum","sequence":"first","affiliation":[{"name":"Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel"},{"name":"Department of Biochemistry and the Chanock Center for Virology, IMRIC, the Hebrew University Faculty of Medicine, Jerusalem, Israel"},{"name":"The Lautenberg Center for General and Tumor Immunology, IMRIC, the Hebrew University Faculty of Medicine, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Oiknine-Djian","sequence":"additional","affiliation":[{"name":"Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel"},{"name":"Department of Biochemistry and the Chanock Center for Virology, IMRIC, the Hebrew University Faculty of Medicine, Jerusalem, Israel"},{"name":"The Lautenberg Center for General and Tumor Immunology, IMRIC, the Hebrew University Faculty of Medicine, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichria","family":"Zakay-Rones","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and the Chanock Center for Virology, IMRIC, the Hebrew University Faculty of Medicine, Jerusalem, 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