{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:52:14Z","timestamp":1791251534602,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2009,12,15]],"date-time":"2009-12-15T00:00:00Z","timestamp":1260835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Rift Valley fever virus<\/jats:italic>\n            (RVFV) (genus\n            <jats:italic>Phlebovirus<\/jats:italic>\n            , family\n            <jats:italic>Bunyaviridae<\/jats:italic>\n            ) causes mosquito-borne epidemic diseases in humans and livestock. The virus carries three RNA segments, L, M, and S, of negative or ambisense polarity. L protein, an RNA-dependent RNA polymerase, encoded in the L segment, and N protein, encoded in the S segment, exert viral RNA replication and transcription. Coexpression of N, hemagglutinin (HA)-tagged L, and viral minigenome resulted in minigenome replication and transcription, a finding that demonstrated HA-tagged L was biologically active. Likewise L tagged with green fluorescent protein (GFP) was biologically competent. Coimmunoprecipitation analysis using extracts from cells coexpressing HA-tagged L and GFP-tagged L showed the formation of an L oligomer. Bimolecular fluorescence complementation analysis and coimmunoprecipitation studies demonstrated the formation of an intermolecular L-L interaction through its N-terminal and C-terminal regions and also suggested an intramolecular association between the N-terminal and C-terminal regions of L protein. A biologically inactive L mutant, in which the conserved signature SDD motif was replaced by the amino acid residues GNN, exhibited a dominant negative phenotype when coexpressed with wild-type L in the minigenome assay system. Expression of this mutant L also inhibited viral gene expression in virus-infected cells. These data provided compelling evidence for the importance of oligomerization of RVFV L protein for its polymerase activity.\n          <\/jats:p>","DOI":"10.1128\/jvi.01310-09","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T00:46:13Z","timestamp":1254962773000},"page":"12779-12789","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Rift Valley Fever Virus L Protein Forms a Biologically Active Oligomer"],"prefix":"10.1128","volume":"83","author":[{"given":"Aya","family":"Zamoto-Niikura","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Terasaki","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Ikegami","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"},{"name":"Pathology"},{"name":"the Sealy Center for Vaccine Development"},{"name":"the Center for Biodefense and Emerging Infectious Diseases, the University of Texas Medical Branch at Galveston, Galveston, Texas 77555"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Peters","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"},{"name":"Pathology"},{"name":"the Sealy Center for Vaccine Development"},{"name":"the Center for Biodefense and Emerging Infectious Diseases, the University of Texas Medical Branch at Galveston, Galveston, Texas 77555"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Makino","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"},{"name":"the Sealy Center for Vaccine Development"},{"name":"the Center for Biodefense and Emerging Infectious Diseases, the University of Texas Medical Branch at Galveston, Galveston, Texas 77555"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-002-0913-4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.234.7.883"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.18.6409"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.07.002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.9.3886"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2005.12.002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01012-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(97)00261-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00220-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02300-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.5.1153"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt816"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.9.5606-5615.2005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.6.2933-2940.2006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2003.tb03424.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.3.1396-1404.1993"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-9-2235"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83387-0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000462"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.09.046"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.18.11974-11980.2005"},{"key":"e_1_3_2_23_2","unstructured":"Rift Valley fever. 1989"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1367209"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-6-1345"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0102-87"},{"key":"e_1_3_2_27_2","unstructured":"Bunyaviruses 2001"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501976102"},{"key":"e_1_3_2_29_2","first-page":"466","volume":"1","year":"1995","unstructured":"Pata, J. D., S. C. Schultz, and K. Kirkegaard. 1995. Functional oligomerization of poliovirus RNA-dependent RNA polymerase. RNA1:466-477.","journal-title":"RNA"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.52.1.215-222.1984"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08565.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1113"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.11.7262-7268.2005"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.007567-0"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)85016-9"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705181105"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1720"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.09.045"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.11.070"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.09.009"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01310-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01310-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:41:49Z","timestamp":1646484109000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01310-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,15]]},"references-count":39,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2009,12,15]]}},"alternative-id":["10.1128\/JVI.01310-09"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01310-09","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,15]]},"assertion":[{"value":"2009-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-09-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}