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In the present work the long-term interactions of immune cells in brains of monkeys were imaged in situ 9 months after the viral inoculation. After viral immunity, the persistent infiltration of T cells and B cells was observed at the infection sites. T cells showed evidence of T-cell receptor signaling as a result of contacts with B cells. Three-dimensional analysis of B-cell-T-cell synapses showed clusters of CD3 in T cells and the segregation of CD20 in B cells, involving the recruitment of CD40 ligand at the interface. These results demonstrate that immunological synapses between B cells and T cells forming three-dimensional microclusters occur in vivo in the central nervous system and suggest that these interactions may be involved in the lymphocyte activation after viral immunity at the original infection site.<\/jats:p>","DOI":"10.1128\/jvi.01326-08","type":"journal-article","created":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T00:46:33Z","timestamp":1218069993000},"page":"9978-9993","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["CD20, CD3, and CD40 Ligand Microclusters Segregate Three-Dimensionally In Vivo at B-Cell-T-Cell Immunological Synapses after Viral Immunity in Primate Brain"],"prefix":"10.1128","volume":"82","author":[{"given":"Carlos","family":"Barcia","sequence":"first","affiliation":[{"name":"Clinical and Experimental Neuroscience"},{"name":"Centro de Investigacio\u0301n Biome\u0301dica en Red 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