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Here, we investigated the initial steps of LCMV infection. We found that the attachment of LCMV to its cellular receptor \u03b1-dystroglycan occurs rapidly and is not dependent on membrane cholesterol. However, subsequent virus internalization is sensitive to cholesterol depletion, indicating the involvement of a cholesterol-dependent pathway. We provide evidence that LCMV entry involves an endocytotic pathway that is independent of clathrin and caveolin and that does not require the GTPase dynamin. In addition, neither the structural integrity nor the dynamics of the actin cytoskeleton are required for infection. These findings indicate that the prototypic Old World arenavirus LCMV uses a mechanism of entry that is different from clathrin-mediated endocytosis, which is used by the New World arenavirus Junin virus, and pathways used by other enveloped viruses.<\/jats:p>","DOI":"10.1128\/jvi.01331-07","type":"journal-article","created":{"date-parts":[[2007,11,29]],"date-time":"2007-11-29T01:44:34Z","timestamp":1196300674000},"page":"1505-1517","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Cellular Entry of Lymphocytic Choriomeningitis Virus"],"prefix":"10.1128","volume":"82","author":[{"given":"Jillian M.","family":"Rojek","sequence":"first","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar","family":"Perez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaciones Oncolo\u0301gicas, Melchor Ferna\u0301ndez Almagro, 3, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kunz","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.521"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02814"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.17.9542-9552.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.9.1303"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.5.1055"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.12.7270-7281.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1001"},{"key":"e_1_3_2_9_2","first-page":"1791","year":"2007","unstructured":"Buchmeier, M. 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