{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:41:10Z","timestamp":1762252870883,"version":"3.41.2"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T00:00:00Z","timestamp":1550188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI067391"],"award-info":[{"award-number":["AI067391"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32AI118689"],"award-info":[{"award-number":["T32AI118689"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI117105"],"award-info":[{"award-number":["AI117105"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI099712"],"award-info":[{"award-number":["AI099712"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,2,15]]},"abstract":"<jats:p>Previous studies have shown that the vaccinia virus glycoproteins A34 and B5 interact, and in the absence of A34, B5 is mislocalized and not incorporated into extracellular virions. Here, using a transient-transfection assay, residues 80 to 130 of the ectodomain of A34 were determined to be sufficient for interaction with B5. Recombinant viruses expressing A34 with a full, partial, or no B5 interaction site were constructed and characterized. All of the A34 truncations interacted with B5 as predicted by the transient-transfection studies but had a small-plaque phenotype. Further analysis revealed that all of the recombinants incorporated detectable levels of B5 into released virions but were defective in cell binding and extracellular virion (EV) dissolution. This study is the first to directly demonstrate that A34 is involved in cell binding and implicate the ectodomain in this role.<\/jats:p>","DOI":"10.1128\/jvi.01343-18","type":"journal-article","created":{"date-parts":[[2018,11,16]],"date-time":"2018-11-16T13:25:45Z","timestamp":1542374745000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["The Ectodomain of the Vaccinia Virus Glycoprotein A34 Is Required for Cell Binding by Extracellular Virions and Contains a Large Region Capable of Interaction with Glycoprotein B5"],"prefix":"10.1128","volume":"93","author":[{"given":"Stephanie R.","family":"Monticelli","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amalia K.","family":"Earley","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Tate","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6703-924X","authenticated-orcid":false,"given":"Brian M.","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010199"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006594107"},{"key":"e_1_3_2_4_2","first-page":"2849","volume-title":"Fields virology","author":"Moss B","year":"2001","unstructured":"Moss B. 2001. Poxviridae: the viruses and their replication, p 2849\u20132883. In Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (ed), Fields virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, PA.","edition":"4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-13-1-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.1503-1517.1999"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.6.4.519-533.1970"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-1-47"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.15.3932"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.1.130-147.1994"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01114-16"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.10.4802-4813.2001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200104124"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.7.4170-4179.1992"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-1-89"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-12-2915"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.6.3753-3762.1996"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.3.1610-1621.1992"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0260"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(86)90309-0"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.8.4732-4741.1993"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.24.11654-11662.2000"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.11.5910-5920.1991"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90591-C"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00037-08"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-1-195"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.12.7217-7224.1992"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.10.5978-5987.1995"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.030460-0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1542"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00249-12"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00598-06"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.4.2863-2875.1999"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01969-07"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90313-E"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01296.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01364-08"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.11.012"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1302"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9483"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.3.2429-2438.1998"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.6.3319-3325.1993"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.1.272-281.1996"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01971-07"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.049486-0"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.020677-0"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.009092-0"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.23.11651-11663.2001"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.5.3904-3915.1997"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.5.4192-4204.1998"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9103"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.03.017"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601025103"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509239102"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.8.4744-4754.2005"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.15.9458-9469.2005"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.17.10988-10998.2005"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.1.303-310.2001"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00253-12"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.27.1.28-37.1978"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/44860"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.05031.x"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(99)00009-3"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80151-8"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90694-A"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.6.2765"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02247-09"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.21.8122"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.10.026"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02154-17"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01343-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01343-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T14:54:14Z","timestamp":1646492054000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01343-18"}},"subtitle":[],"editor":[{"given":"Rozanne M.","family":"Sandri-Goldin","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,2,15]]},"references-count":69,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,2,15]]}},"alternative-id":["10.1128\/JVI.01343-18"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01343-18","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"type":"print","value":"0022-538X"},{"type":"electronic","value":"1098-5514"}],"subject":[],"published":{"date-parts":[[2019,2,15]]},"assertion":[{"value":"2018-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01343-18"}}